Structure of PDB 5f9f Chain K

Receptor sequence

>5f9fK (length=672) Species: 9606 (Homo sapiens)

KPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKG
KVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENN
DIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYL
DQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIAT
VKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICK
DLREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYN
DALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELES
VSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEG
NPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSV
ADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIE
KEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPVP
DKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPK
QFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGV
QTLYSKWKDFHFEKIPFDPAEM

3D structure

• PDB: 5f9f Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I.
• Chain: K
• Resolution: 2.601 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	K	N298 I300 S325 G326 T347 Q349 K379 Q380 H381 Q507 E510 Q511 K518 R546 K635 T636 R637 T662 G663 R664 T667 N668 T697 S698 N720 K750 H830 K851 F853 K858 I875 V886 K888 W908	N57 I59 S84 G85 T106 Q108 K138 Q139 H140 Q258 E261 Q262 K269 R297 K386 T387 R388 T413 G414 R415 T418 N419 T448 S449 N471 K501 H579 K600 F602 K607 I624 V635 K637 W657	PDBbind-CN: Kd=38.5nM
BS02	ZN	K	C810 C864	C559 C613
BS03	BU3	K	I262 A264 K270 V273 L408 T409	I21 A23 K29 V32 L167 T168

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5f9f, PDBe:5f9f, PDBj:5f9f
• PDBsum: 5f9f
• PubMed: 26733676
• UniProt: O95786|RIGI_HUMAN Antiviral innate immune response receptor RIG-I (Gene Name=RIGI)