Structure of PDB 7aju Chain JP

Receptor sequence

>7ajuJP (length=461) Species: 559292 (Saccharomyces cerevisiae S288C)

MKIKTIKRSADDYVPVKSTQESQMPRNLNPELHPFERAREYTKALNATKL
ERMFAKPFVGQLGYGHRDGVYAIAKNYGSLNKLATGSADGVIKYWNMSTR
EEFVSFKAHYGLVTGLCVTQPRFHDKKPDLKSQNFMLSCSDDKTVKLWSI
NVDDYSNKNNEEGLIRTFDGESAFQGIDSHRENSTFATGGAKIHLWDVNR
LKPVSDLSWGADNITSLKFNQNETDILASTGSDNSIVLYDLRTNSPTQKI
VQTMRTNAICWNPMEAFNFVTANEDHNAYYYDMRNLSRSLNVFKDHVSAV
MDVDFSPTGDEIVTGSYDKSIRIYKTNHGHSREIYHTKRMQHVFQVKYSM
DSKYIISGSDDGNVRLWRSKAWERSNVKTTREKNKLEYDEKLKERFRHMP
EIKRISRHRHVPQVIKKAQEIKNIELSSIKRREANERRTRKDMPYISERK
KQIVGTVHKYE

3D structure

• PDB: 7aju Structure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome.
• Chain: JP
• Resolution: 3.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	JP	K425 E433 S436 R439 R440 N443 R446 T447 K449	K417 E425 S428 R431 R432 N435 R438 T439 K441
BS02	rna	JP	S18 E21 R52 M53 F54 K56 P57 F58 E341 H344 T345 K346 R347 R373 R382 T387 R389 E390 K393 L394 H418	S18 E21 R52 M53 F54 K56 P57 F58 E333 H336 T337 K338 R339 R365 R374 T379 R381 E382 K385 L386 H410

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7aju, PDBe:7aju, PDBj:7aju
• PDBsum: 7aju
• PubMed: 33326748
• UniProt: P33750|DCA13_YEAST Protein SOF1 (Gene Name=SOF1)