Structure of PDB 8hsg Chain J

Receptor sequence

>8hsgJ (length=1278) Species: 300852 (Thermus thermophilus HB8)

KKEVRKVRIALASPEKIRSWSYGEVEKPETINYRTLKPERDGLFDERIFG
PIKDTKSIVRRYRMGHIELATPAAHIWFVKDVPSKIGTLLDLSATELEQV
LYFSKYIVLDPKGAILNGVPVEKRQLLTDEEYRELRYGKQETYPLPPGVD
ALVKDGEEVVKGQELAPGVVSRLDGVALYRFASILVVKARVYPFEDDVEV
STGDRVAPGDVLADGGKVKSDVYGRVEVDLVRNVVRVVESYDIDARMGAE
AIQQLLKELDLEALEKELLEEMKHPSRARRAKARKRLEVVRAFLDSGNRP
EWMILEAVPVLPPDLRPMVQVDGGRFATSDLNDLYRRLINRNNRLKKLLA
QGAPEIIIRNEKRMLQEAVDALLDNGRRGAPVTNPGSDRPLRSLTDILSG
KQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKRMALELFKPFLLK
KMEEKGIAPNVKAARRMLERQRDIKDEVWDALEEVIHGKVVLLNRAPTLH
RLGIQAFQPVLVEGQSIQLHPLVCEAFNADFDGDQMAVHVPLSSFAQAEA
RIQMLSAHNLLSPASGEPLAKPSRDIILGLYYITQVRKEKKGAGLEFATP
EEALAAHERGEVALNAPIKVAGRETSVGRLKYVFANPDEALLAVAHGIVD
LQDVVTVRYMGKRLETSPGRILFARIVAEAVEDEKVAWELIQLDVPQEKN
SLKDLVYQAFLRLGMEKTARLLDALKYYGFTFSTTSGITIGIDDAVIPEE
KKQYLEEADRKLLQIEQAYEMGFLTDRERYDQILQLWTETTEKVTQAVFK
NFEENYPFNPLYVMAQSGARGNPQQIRQLCGLRGLMQKPSGETFEVPVRS
SFREGLTVLEYFISSHGARKGGADTALRTADSGYLTRKLVDVTHEIVVRE
ADCGTTNYISVPLFQPDEVTRSLRLRKRADIEAGLYGRVLAREVEVLGVR
LEEGRYLSMDDVHLLIKAAEAGEIQEVPVRSPLTCQTRYGVCQKCYGYDL
SMARPVSIGEAVGIVAAQSIGEPGTQLGLPRVIELFEARRPKAKAVISEI
DGVVRIEETEEKLSVFVESEGFSKEYKLPKEARLLVKDGDYVEAGQPLTR
GAIDPHQLLEAKGPEAVERYLVEEIQKVYRAQGVKLHDKHIEIVVRQMMK
YVEVTDPGDSRLLEGQVLEKWDVEALNERLIAEGKTPVAWKPLLMGVTKS
ALSTKSWLSAASFQNTTHVLTEAAIAGKKDELIGLKENVILGRLIPAGTG
SDFVRFTQVVDQKTLKAIEEARKEAVEA

3D structure

• PDB: 8hsg Structural basis of the transcription termination factor Rho engagement with transcribing RNA polymerase from Thermus thermophilus.
• Chain: J
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	J	R486 R488 R534 N593 K610 K621 R628 A705 A1089 Y1093 Q1441	R277 R279 R325 N384 K401 K412 R419 A496 A880 Y884 Q1214
BS02	rna	J	V528 R704 N737 D739 D741 D743	V319 R495 N528 D530 D532 D534
BS03	dna	J	N593 R1266 K1426	N384 R1039 K1199
BS04	ZN	J	C1194 C1201 C1204	C985 C992 C995
BS05	MG	J	D739 D741 D743	D530 D532 D534

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:8hsg, PDBe:8hsg, PDBj:8hsg
• PDBsum: 8hsg
• PubMed: 36753546
• UniProt: Q8RQE8|RPOC_THET8 DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)