Structure of PDB 6xas Chain J

Receptor sequence
>6xasJ (length=1327) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
AQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCA
RIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELAS
PTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQI
LTEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETN
SETKRKKLTKRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGR
FATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDN
GRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGP
YLRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVVW
DILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNA
DFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVLG
LYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDA
NGELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCY
RILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEA
EVAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINR
DGQEEKQVSFNSIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPI
TANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVV
TEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADILVP
RNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHII
NKGEAIGVIAAQSIGEPGTQLTMRSSIQVKNKGSIKLSNVKSVVNSSGKL
VITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPH
TMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKD
LRPALKIVDAQYFLPGKAIVQLEDGVQISSGDTLARIPQGGLPRVADLFE
ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQL
NVFEGERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGV
KINDKHIEVIVRQMLRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANG
KVGATYSRDLLGITKASLATESFISAASFQETTRVLTEAAVAGKRDELRG
LKENVIVGRLIPAGTGYAYHQDRMRRR
3D structure
PDB6xas Pre-termination Transcription Complex: Structure and Function.
ChainJ
Resolution3.8 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna J R133 R314 K1170 R124 R305 K1124
BS02 dna J S210 E211 T212 S319 R322 R339 R346 A791 Y795 S201 E202 T203 S310 R313 R330 R337 A782 Y786
BS03 MG J D460 D464 D451 D455
BS04 ZN J C85 C88 C76 C79
BS05 ZN J C814 C888 C898 C805 C879 C889
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006879 intracellular iron ion homeostasis
GO:0009408 response to heat
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0036460 cellular response to cell envelope stress
GO:0042128 nitrate assimilation
GO:0044780 bacterial-type flagellum assembly
GO:0046677 response to antibiotic
GO:0048870 cell motility
GO:0071973 bacterial-type flagellum-dependent cell motility
GO:0090605 submerged biofilm formation
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0000428 DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008023 transcription elongation factor complex
GO:0016020 membrane

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:6xas, PDBe:6xas, PDBj:6xas
PDBsum6xas
PubMed33296676
UniProtP0A8T7|RPOC_ECOLI DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)

[Back to BioLiP]