Structure of PDB 5gan Chain J

Receptor sequence

>5ganJ (length=729) Species: 4932 (Saccharomyces cerevisiae)

GNVDLNKLTEEREKLLQSQIDENLAQLTKNASNPIQVNKPNAATDALSYL
KDLENDRVNSLSDATLEDLQKMRTILKSYRKADPTNPQGWIASARLEEKA
RKFSVAKKIIENGCQECPRSSDIWLENIRLHESDVHYCKTLVATAINFNP
TSPLLWFKAIDLESTTVNKYRVVRKALQEIPRDEGLWKLAVSFEADKAQV
IKMLEKATQFIPQSMDLLTAYTNLQSYHNAKMTLNSFRKILPQEPEIWII
STLLEERNNPKLVSLLKEGLLELSKNGYKATLSAWLKRAEALNDAPNSNL
TCQAIVYAILEWLRESGEYESELNNVDQILEKMPHSKVQIAVLKKLIQWD
PCDTVLWSRLKMATESYEELLAFFQELLFQTSDDIRANMREKSPGLLMMY
VSEYWKAQKGDTRQTLVLIDQIIDFAPHNLDLRFFKIKLLGRSLQLDELR
DFFQQTFSSLEDFKISGTERLYYKYVNFLRYQDLNEEAIKFLNERCLKSF
PICHKFFLQLGQIYHSMGNIEMSRETYLSGTRLVPNCPLLWVSLSKIDEI
DLKNPVRARSILDRGLLKNPDDVLFYIAKIQMEIRLGNLDQAELLVTQAL
QKFPSNALLWVEQIKLFKHGNKSSLKKTIFQDALRRTQNDHRVLLEIGVS
FYAEAQYETSLKWLERALKKCSRYGDTWVWLFRTYARLGKDTVDLYNMFD
QCEPTYGPEWIAASKNCTPREILLRLMND

3D structure

• PDB: 5gan Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 angstrom resolution.
• Chain: J
• Resolution: 3.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	J	P188 I189 A196 A197 D199 A200 L201 Y203 S788 K791	P34 I35 A42 A43 D45 A46 L47 Y49 S624 K627

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding

Biological Process

• GO:0000244 spliceosomal tri-snRNP complex assembly
• GO:0000398 mRNA splicing, via spliceosome
• GO:0006396 RNA processing
• GO:0006397 mRNA processing
• GO:0008150 biological_process
• GO:0008380 RNA splicing

Cellular Component

• GO:0005634 nucleus
• GO:0005681 spliceosomal complex
• GO:0005682 U5 snRNP
• GO:0046540 U4/U6 x U5 tri-snRNP complex
• GO:0071001 U4/U6 snRNP
• GO:0071013 catalytic step 2 spliceosome

External links

• PDB: RCSB:5gan, PDBe:5gan, PDBj:5gan
• PDBsum: 5gan
• PubMed: 26829225
• UniProt: P19735|PRP6_YEAST Pre-mRNA-splicing factor 6 (Gene Name=PRP6)