Structure of PDB 4ilm Chain J

Receptor sequence

>4ilmJ (length=283) Species: 273057 (Saccharolobus solfataricus P2)

MPLIFKIGYNVIPLQDVILPTPSSKVLKYLIQSGKLIPSLKDLITSRDKY
KPIFISHLGFNQRRIFQTNKTITKGSRLSSIIAFSTQANVLSEVADEGIF
ETVYGKFHIMIESIEIVEVEKLKEEVEKHMNDNIRVRFVSPTLLSSKVLL
PPSLSERYKKIHAGYSTLPSVGLIVAYAYNVYCNLIGKKEVEVRAFKFGI
LSNALSRIIGYDLHPVTVAIGEDSKGNLRKARGVMGWIEFDIPDERLKRR
ALNYLLTSSYLGIGRSRGIGFGEIRLEFRKIEE

3D structure

• PDB: 4ilm Recognition and cleavage of a nonstructured CRISPR RNA by its processing endoribonuclease Cas6.
• Chain: J
• Resolution: 3.068 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	J	N136 R210 I212 G213 Y214 W240 D244 R282	N133 R207 I209 G210 Y211 W237 D241 R279
BS02	rna	J	K28 R47 D48 K49 Y50 K51 S148 K150 V151 L153 G167 Y168 Y180 H217 T220 I223 S227 R232 K233 A234 R235 R268 S269 G271 I272	K28 R47 D48 K49 Y50 K51 S145 K147 V148 L150 G164 Y165 Y177 H214 T217 I220 S224 R229 K230 A231 R232 R265 S266 G268 I269

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0016788 hydrolase activity, acting on ester bonds

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:4ilm, PDBe:4ilm, PDBj:4ilm
• PDBsum: 4ilm
• PubMed: 23454186
• UniProt: Q97WV8|CAS6B_SACS2 CRISPR-associated endoribonuclease Cas6 2 (Gene Name=cas6b)