Structure of PDB 4gd3 Chain J

Receptor sequence
>4gd3J (length=581) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
STQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVITNAVSCGT
MFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPD
NANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQS
LSSWPKSSPGYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLM
GFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPCAINIDESGAVGA
VNMERLNLVQSIITRTADFINNVMIPDALAIGQFNKPWSEIGTGLSDKCV
LSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVDLVDPQQVQEFVDH
AWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAP
RWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLGR
ILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRG
VGFTEAPRGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAA
LMNTKMAIPEQPLEILRTLHSFDPCLACSTH
3D structure
PDB4gd3 Crystal Structure of the O(2)-Tolerant Membrane-Bound Hydrogenase 1 from Escherichia coli in Complex with Its Cognate Cytochrome b.
ChainJ
Resolution3.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E28 C76 C79 H83
Catalytic site (residue number reindexed from 1) E27 C75 C78 H82
Enzyme Commision number 1.12.99.6: hydrogenase (acceptor).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FCO J C79 V82 H83 A507 P508 R509 V530 T532 C576 C579 C78 V81 H82 A506 P507 R508 V529 T531 C575 C578
BS02 MG J E57 C528 H582 E56 C527 H581
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008901 ferredoxin hydrogenase activity
GO:0009055 electron transfer activity
GO:0016151 nickel cation binding
GO:0016491 oxidoreductase activity
GO:0033748 hydrogenase (acceptor) activity
GO:0046872 metal ion binding
Biological Process
GO:0006113 fermentation
GO:0009061 anaerobic respiration
GO:0009267 cellular response to starvation
GO:0019645 anaerobic electron transport chain
GO:1902421 hydrogen metabolic process
Cellular Component
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0030288 outer membrane-bounded periplasmic space
GO:0044569 [Ni-Fe] hydrogenase complex
GO:0098567 periplasmic side of plasma membrane

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Molecular Function
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Cellular Component
External links
PDB RCSB:4gd3, PDBe:4gd3, PDBj:4gd3
PDBsum4gd3
PubMed23260654
UniProtP0ACD8|MBHL_ECOLI Hydrogenase-1 large chain (Gene Name=hyaB)

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