Structure of PDB 2eq9 Chain J

Receptor sequence

>2eq9J (length=460) Species: 300852 (Thermus thermophilus HB8)

MKTYDLIVIGTGPGGYHAAIRAAQLGLKVLAVEAGEVGGVCLNVGCIPTK
ALLHAAETLHHLKVAEGFGLKAKPELDLKKLGGWRDQVVKKLTGGVGTLL
KGNGVELLRGFARLVGPKEVEVGGERYGAKSLILATGSEPLELKGFPFGE
DVWDSTRALKVEEGLPKRLLVIGGGAVGLELGQVYRRLGAEVTLIEYMPE
ILPQGDPETAALLRRALEKEGIRVRTKTKAVGYEKKKDGLHVRLEPAEGG
EGEEVVVDKVLVAVGRKPRTEGLGLEKAGVKVDERGFIRVNARMETSVPG
VYAIGDAARPPLLAHKAMREGLIAAENAAGKDSAFDYQVPSVVYTSPEWA
GVGLTEEEAKRAGYKVKVGKFPLAASGRALTLGGAEGMVKVVGDEETDLL
LGVFIVGPQAGELIAEAALALEMGATLTDLALTVHPHPTLSESLMEAAEA
FHKQAIHILN

3D structure

• PDB: 2eq9 Crystal structure of lipoamide dehydrogenase from Thermus thermophilus HB8
• Chain: J
• Resolution: 2.09 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): V43 C47 C52 T55 A186 E190 H444 H446 E451 N469
• Catalytic site (residue number reindexed from 1): V37 C41 C46 T49 A176 E180 H435 H437 E442 N460
• Enzyme Commision number: 1.8.1.4: dihydrolipoyl dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	J	I15 G16 G18 P19 G20 V38 E39 A40 G45 V46 C47 G51 C52 F118 A119 A146 T147 G148 S166 R274 R277 G313 D314 L320 L321 A322 H323 Y353	I9 G10 G12 P13 G14 V32 E33 A34 G39 V40 C41 G45 C46 F111 A112 A135 T136 G137 S155 R266 R269 G305 D306 L312 L313 A314 H315 Y344

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004148 dihydrolipoyl dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
• GO:0050660 flavin adenine dinucleotide binding

External links

• PDB: RCSB:2eq9, PDBe:2eq9, PDBj:2eq9
• PDBsum: 2eq9
• UniProt: Q5SLR0