Structure of PDB 8ow1 Chain II

Receptor sequence

>8ow1II (length=704) Species: 4932 (Saccharomyces cerevisiae)

MSLILDDIILSLTNANERTPPQALKTTLSLLYEKSKQYGLSSPQLQALVR
LLCETSIIDTVTKVYIVENCFLPDGYLTKELLLEIINHLGTPTVFSRYRI
QTPPVLQSALCKWLVHVYFLFPVHSEREHNISSSIWLHLWQFSFLQKWIT
PLVIWQATTPVDVKPWKLSIIKRCAMHPGYRDAPGSATLILQRFQCLVGA
SSQITESIITINCNRKTLKSHRNLKLDAHFLSILKRILSRAHPANFPADT
VQNTIDMYLSEIHQLGADDSTVSLWDVTSLEQLAQNWPQLHIPNDVDYMM
KPRKVMSRDSLKHLYSSIILIKNSRDESSSPYEWCIWQLKRCFAHQIETP
QEVIPIIISVSSMDNKLSSRIIQTFCNLKYLKLDELTLKKVCGGILPLWK
PELISGTREFFVKFMASIFMWSTRDGNNCTFSETCFYVLQMITNWVLDDK
LIALGLTLLHDMQSLLTLDKIFNNATSNRFSTMAFISSLDILTQLSKQTK
SDYAIQYLIVGPDIMNKVFSSDDPLLLSAACRYLVATKNKLMQYPSTNKF
VRMQNQYIMDLTNYLYRNKVLSSKSLFGVSPDFFKQILENLYIPTADFKN
AKFFTITGIPALSYICIIILRRLETAENTKIKFTSGIINEETFNNFFRVH
HDEIGQHGWIKGVNNIHDLRVKILMHLSNTANPYRDIAAFLFTYLKSLSK
YSVQ

3D structure

• PDB: 8ow1 Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.
• Chain: II
• Resolution: 3.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	II	N214 R215 K216 K219	N214 R215 K216 K219

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding

Biological Process

• GO:0006270 DNA replication initiation
• GO:0007059 chromosome segregation
• GO:0007094 mitotic spindle assembly checkpoint signaling
• GO:0008608 attachment of spindle microtubules to kinetochore
• GO:0034087 establishment of mitotic sister chromatid cohesion
• GO:0051301 cell division
• GO:0051321 meiotic cell cycle

Cellular Component

• GO:0000775 chromosome, centromeric region
• GO:0000776 kinetochore
• GO:0005634 nucleus
• GO:0005694 chromosome

External links

• PDB: RCSB:8ow1, PDBe:8ow1, PDBj:8ow1
• PDBsum: 8ow1
• PubMed: 37506202
• UniProt: Q12748|CENPI_YEAST Inner kinetochore subunit CTF3 (Gene Name=CTF3)