Structure of PDB 3jai Chain II

Receptor sequence

>3jaiII (length=206) Species: 9986 (Oryctolagus cuniculus)

GISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGG
NKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCI
VLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDE
RKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRK
IKARKG

3D structure

• PDB: 3jai Structural basis for stop codon recognition in eukaryotes.
• Chain: II
• Resolution: 3.65 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	II	R77 I79 D80 E89 R92 L103 Q168 G169 K170	R76 I78 D79 E88 R91 L102 Q167 G168 K169
BS02	rna	II	G2 I3 R5 N7 H9 K10 R11 R12 K13 T14 G15 G16 K17 K19 Y21 H22 K23 K24 R25 K26 Y27 L29 R31 A34 R41 R42 I43 H44 R47 V48 R49 G50 N52 K53 K54 Y55 R56 L58 N64 T73 R74 S86 N87 V97 K98 N99 R141 K147 A176 R178 Q181 C182 R184 D186 G187	G1 I2 R4 N6 H8 K9 R10 R11 K12 T13 G14 G15 K16 K18 Y20 H21 K22 K23 R24 K25 Y26 L28 R30 A33 R40 R41 I42 H43 R46 V47 R48 G49 N51 K52 K53 Y54 R55 L57 N63 T72 R73 S85 N86 V96 K97 N98 R140 K146 A175 R177 Q180 C181 R183 D185 G186

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3jai, PDBe:3jai, PDBj:3jai
• PDBsum: 3jai
• PubMed: 26245381
• UniProt: G1TJW1|RS8_RABIT Small ribosomal subunit protein eS8 (Gene Name=RPS8)