Structure of PDB 7pua Chain IA

Receptor sequence

>7puaIA (length=704) Species: 5702 (Trypanosoma brucei brucei)

QSQSSRGQPGLQWQEGVVDPRYSSSGNMRRFNPDTVAHYVKATIQNDRRE
MGLGEVYDWHEFAKDAVYIPTRSGPLWVGSDDPRCAKFMRRRSKMQKTPQ
QKARPKPGPDPAKALEDHPLREYFTTATNLRDPLSTANNLHRAGLIREYD
IKYTAAKVRYVPRPPVVSIMGHVDHGKTTLLDYLRKTNVASQEAGGITQN
VGAFQVKTLGDTLVTFIDTPGHAAFTTMREVGATANDLIVLVVSAVDGVQ
PQTKEVIELAHKSGIPFVVACTKIDRQPRVENVKQQLRDCNVELEEDGGD
TQFVPVCARDGRGVPELLEAIALQAELCEISTPKPSRCEVTVIETNGVNT
TTEVAGIVRCGKVRAGQVLVAGMTYAVVRKVLDEHGQIIQEAGPSQPVVL
HGFRVHPKPGSILLQVSSESHAQKFYHFMKEVYQAEGRREDYLQLLNQEQ
HGMLYARKPDNNLVRTYSTQAFVLSCKAATFGMLQALMKSIYEIPRLEGI
STEIKVTEVGGLRDYDVALIGSSGQPGCILLYGGCKDTNTLDVPNHVTVI
RFNVLYHGLEALKETLVGALPKITKIRVTAEAECLQVFRASQAGKSGNAG
GMRVTKGTIIAAHLTFRVCRKAPGHRVVVYEGQIKELRRFKELVPSVELG
LECGVIMQDEFQFRTGDILQQYETYEEPRDVAEEYQKAELREKIMRDTAA
AEAL

3D structure

• PDB: 7pua Mitoribosomal small subunit maturation involves formation of initiation-like complexes.
• Chain: IA
• Resolution: 3.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	IA	Q40 R45 Q47 P48 R60 T110 R111 S112 K126 R130 K133 Q140 A142 P144 K145 Y494 K497 P498 N500	Q1 R6 Q8 P9 R21 T71 R72 S73 K87 R91 K94 Q101 A103 P105 K106 Y455 K458 P459 N461
BS02	peptide	IA	V626 F627 R700 F702 K703	V587 F588 R638 F640 K641
BS03	GDP	IA	V212 D213 H214 G215 K216 T217 T218 K312 D314 R348	V173 D174 H175 G176 K177 T178 T179 K273 D275 R309
BS04	MG	IA	T217 T237	T178 T198

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003743 translation initiation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding

Biological Process

• GO:0006412 translation
• GO:0006413 translational initiation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion

External links

• PDB: RCSB:7pua, PDBe:7pua, PDBj:7pua
• PDBsum: 7pua
• PubMed: 35042777
• UniProt: C9ZSN7