Structure of PDB 8tfk Chain I

Receptor sequence

>8tfkI (length=440) Species: 192952 (Methanosarcina mazei Go1)

TTKEDVLEAVKERDVKFIRTQFTDTLGIIKSWAIPAEQLEEAFENGVMFD
GSSIQGFTRIEESDMKLALDPSTFRILPWRPATGAVARILGDVYLPDGNP
FKGDPRYVLKTAIKEAEKMGFSMNVGPELEFFLFKLDANGNPTTELTDQG
GYFDFAPLDRAQDVRRDIDYALEHMGFQIEASHHEVAPSQHEIDFRFGDV
LCTADNVVTFKYVVKSIAYHKGYYASFMPKPLFGVNGSGMHSNQSLFKDG
KNVFYDPDTPTKLSQDAMYYIGGLLKHIREFTAVTNPVVNSYKRLVPGYE
APVYISWSAQNRSSLIRIPATRGNGTRIELRCPDPACNPYLAFALMLRAG
LEGIKNKIDPGEPTNVNIFHLSDKEREERGIRSLPADLKEAIDEMKGSKF
VKEALGEHVFSHYLCAKEMEWDEYKAVVHPWELSRYLSML

3D structure

• PDB: 8tfk M. mazei glutamine synthetase and glutamine synthetase-GlnK1 structures reveal enzyme regulation by oligomer modulation.
• Chain: I
• Resolution: 2.66 Å

Enzymatic activity

• Enzyme Commision number: 6.3.1.2: glutamine synthetase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	P3S	I	E137 E192 E199 G244 H248 R301 E307 R319 R338	E130 E185 E192 G237 H241 R294 E300 R312 R331
BS02	ADP	I	E135 D201 F202 F204 N250 S252 R324 G332 R334 E336	E128 D194 F195 F197 N243 S245 R317 G325 R327 E329
BS03	MG	I	E137 E192 E199	E130 E185 E192
BS04	MG	I	E135 H248 E336	E128 H241 E329
BS05	MG	I	E135 E199	E128 E192

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004356 glutamine synthetase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006542 glutamine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8tfk, PDBe:8tfk, PDBj:8tfk
• PDBsum: 8tfk
• PubMed: 37968329
• UniProt: Q8PY99|GLNA1_METMA Glutamine synthetase (Gene Name=glnA1)