Structure of PDB 8dh5 Chain I

Receptor sequence

>8dh5I (length=837) Species: 10760 (Escherichia phage T7)

NTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARF
RKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPT
AFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARF
GRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAW
SSWHKEDSIHVGVRCIEMLIESTGMVSLHSETIELAPEYAEAIATRAGAL
AGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDV
YMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPKRAAAAVY
RKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQ
GNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEEN
HENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLA
FDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQAD
AINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVM
TLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESV
SVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPV
WQEYKKPIQTRLNLMFLGQDSEIDAHKQESGIAPNFVHSQDGSHLRKTVV
WAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFY
DQFADQLHESQLDKMPALPAKGNLNLRDILESDFAFA

3D structure

• PDB: 8dh5 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.
• Chain: I
• Resolution: 2.85 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	I	R50 R57 E63 K160 K164 R298 W422 R423 Y427 T636 Y639 S641 F644 Y739 H772 P780 H784	R49 R56 E62 K159 K163 R287 W387 R388 Y392 T601 Y604 S606 F609 Y704 H726 P734 H738
BS02	rna	I	N171 K172 R386 K389 R394 R425 G436 I810 H811	N170 K171 R351 K354 R359 R390 G401 I764 H765
BS03	dna	I	E643 R647 G675 K679 K704	E608 R612 G640 K644 K669
BS04	ATP	I	D471 Y571 K631 M635 Y639	D436 Y536 K596 M600 Y604

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039695 DNA-templated viral transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:8dh5, PDBe:8dh5, PDBj:8dh5
• PDBsum: 8dh5
• PubMed: 36635281
• UniProt: P00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)