Structure of PDB 8dh4 Chain I

Receptor sequence
>8dh4I (length=821) Species: 10760 (Escherichia phage T7) [Search protein sequence]
TINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFR
KMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTA
FQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFG
RIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWS
SWHKEDSIHVGVRCIEMLIESTGMVSLHLAPEYAEAIATRAGALAGISPM
FQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVY
KAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPTAWKRAAAAVYRKD
KARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGND
MTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHEN
IMACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDG
SCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAIN
GTDNEVKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVL
EDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKS
AAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMF
LGQDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHD
SFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMP
ALPAKGNLNLRDILESDFAFA
3D structure
PDB8dh4 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.
ChainI
Resolution2.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna I R50 E63 R298 R423 Y427 T636 Y639 F644 Y739 P780 H784 R48 E61 R281 R385 Y389 T585 Y588 F593 Y688 P718 H722
BS02 rna I N171 K389 R394 G436 Y639 I810 N169 K351 R356 G398 Y588 I748
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0019083 viral transcription
GO:0039695 DNA-templated viral transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8dh4, PDBe:8dh4, PDBj:8dh4
PDBsum8dh4
PubMed36635281
UniProtP00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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