Structure of PDB 8dh2 Chain I

Receptor sequence
>8dh2I (length=833) Species: 10760 (Escherichia phage T7) [Search protein sequence]
NTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARF
RKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVTAFQFLQEI
KPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEA
KHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDS
IHVGVRCIEMLIESTGMVSLHTIELAPEYAEAIATRAGALAGISPMFQPC
VVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAIN
IAQNTAWKINKKVLAVANVITKWKHCPVEDIPEALTAWKRAAAAVYRKDK
ARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDM
TKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENI
MACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGS
CSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAING
TDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAY
GSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTV
VAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEY
KKPIQTRLNLMFLGQDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHE
KYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFA
DQLHESQLDKMPALPAKGNLNLRDILESDFAFA
3D structure
PDB8dh2 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.
ChainI
Resolution2.9 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna I R50 R57 E63 R298 R423 Y427 T636 Y639 S641 F644 G645 Q649 Y739 P780 R49 R56 E62 R277 R384 Y388 T597 Y600 S602 F605 G606 Q610 Y700 P730
BS02 rna I N171 K172 R386 S393 R394 N162 K163 R347 S354 R355
BS03 3PO I D471 K472 D432 K433
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0019083 viral transcription
GO:0039695 DNA-templated viral transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8dh2, PDBe:8dh2, PDBj:8dh2
PDBsum8dh2
PubMed36635281
UniProtP00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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