Structure of PDB 8dh1 Chain I

Receptor sequence
>8dh1I (length=825) Species: 10760 (Escherichia phage T7) [Search protein sequence]
IAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMF
ERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKPTAFQFLQEI
KPEAVAYITIKTTLACLTSNTTVQAVASAIGRAIEDEARFGRIRDLEAKH
FKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIH
VGVRCIEMLIESTGMVSLHSETIELAPEYAEAIATRAGALAGISPMFQPC
VVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAIN
IAQNTAWKINKKVLAVANVITKWKHCPVEDIPAKRAAAAVYRKDKARKSR
RISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLL
TLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAK
SPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQ
HFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAINGTDNEV
VTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEF
GFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVE
AMNWLKSAAKLLAAEVKDEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLN
LMFLGQFDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFA
LIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQL
DKMPALPAKGNLNLRDILESDFAFA
3D structure
PDB8dh1 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.
ChainI
Resolution2.65 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna I R50 R57 R298 W422 R423 Y427 R632 T636 Y639 S641 F644 Q649 Y739 P780 H784 R45 R52 R277 W378 R379 Y383 R588 T592 Y595 S597 F600 Q605 Y691 P722 H726
BS02 rna I N171 R386 A390 G436 I810 H811 D812 N160 R342 A346 G392 I752 H753 D754
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0019083 viral transcription
GO:0039695 DNA-templated viral transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8dh1, PDBe:8dh1, PDBj:8dh1
PDBsum8dh1
PubMed36635281
UniProtP00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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