Structure of PDB 7yed Chain I

Receptor sequence

>7yedI (length=1288) Species: 538123 (Mammalian orthoreovirus 3)

ANVWGVRLADSLSSPTIETRTRHYTLHDFYSDLDASVGKEPWRPLRNQRT
NEIVAVQLFRPLQGLVFDTQLYGFPGTFSQWEQFMKEKLRVLKYEVLRIY
PISTYNHDRVNVFVANALVGAFLSNQAFYDLLPLLIVNDTMISDLLGTGA
ALSQFFQSHGEVLEVAAGRKYLQMNNYSNDDDDPPLFAKDLSDYAKAFYS
DTYEVLDRFFWTHDSSAGVLVHYDKPTNGNHYILGTLTQMVSAPPHIINA
TDALLLESCLEQFAANVRARSAQPVTRLDQCYHLRWGAQYVGEDSLTYRL
GVLSLLATNGYQLARPIPKQLTNRWLSSFVSQVVSDGINETPLWPQERYV
QIAYDSPSVVDGATQYGYVRRNQLRLGMRISALQSLSDTPAPVQWLPQYT
IDQVAVDEGDAMVSQLTQLPLRPDYGSIWIGEALSYYVDYNRSHRVVLSS
ELPQLPDTYFDGDEQYGRSLFSLARKVGDRSLVKDTAVLKHAYQAIDPNT
GKEYLRAGQSVAYFGASAGHSGADQPLVIEPWMQGKISGVPPPSSVRQFG
YDVAKGAIVDLARPFPSGDYQFVYSDVDQVVDGHDDLSISSGLVESLLDS
CVHATAPGGSFVMKINFPTRTVWHYIEQKILPNVTSYMLIKPFVTNNVEV
FFVAFGVHQQSALTWTSGVYFFLVDHFYRYETLSAISRQLPSFGYVDDGS
SVTGIEIISIENPGFSNMTQAARVGISGLCANVGNARKSIAIYESHGARV
LTITSRRSPASARRKARLRYLPLIDPRSLEVQARTILPSNPVLFDNINGA
SPHVCLTMMYNFEVSSAVYDGDVVLDLGTGPEAKILELIPSTSPVTCVDI
RPTAQPNGCWNVRTTFLELDYLSDGWITGVRGDIVTCMLSLGAAAAGKSM
TFDAAFQQLVRVLTRSTANVLLIQVNCPTDVIRTIKGYLEIDQTNKRYKF
PKFGRDEPYSDMDSLERICRAAWPNCSITWVPLSYDLRWTKLALLESTTL
SSASVRIAELMYKYMPIMRIDIHGLPMEKQGNFIVGQNCSLVIPGFNAQD
VFNCYFNSALAFSTEDVNSAMIPQVTAQFDANKGEWSLDMVFSDAGIYTM
QALVGSNANPVSLGSFVVDSPDVDITDAWPAQLDFTIAGTDVDITVNPYY
RLMAFVKIDGQWQIANPDKFQFFSSNTGTLVMNVKLDIADRYLLYYIRDV
QSRDVGFYIQHPLQLLNTITLPTNEDLFLSAPDMREWAVKESGNTICILN
SPGFIPPQDWDVLTDTISWSPSLPTYVVPPGDYTLTPL

3D structure

• PDB: 7yed In situ structures of polymerase complex of mammalian reovirus illuminate RdRp activation and transcription regulation.
• Chain: I
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.7.7.50: mRNA guanylyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAM	I	S482 Y514 S518 H521 P527 Y552 L562 D577 V578 D579 V582 D583	S481 Y513 S517 H520 P526 Y551 L561 D576 V577 D578 V581 D582
BS02	GTP	I	N117 K171 Y195 T228 H232	N116 K170 Y194 T227 H231
BS03	SAM	I	G829 T830 D850 I851 D871 Y872 M889 S891 A895	G828 T829 D849 I850 D870 Y871 M888 S890 A894

Gene Ontology

Molecular Function

• GO:0004482 mRNA 5'-cap (guanine-N7-)-methyltransferase activity
• GO:0004484 mRNA guanylyltransferase activity
• GO:0005524 ATP binding
• GO:0005525 GTP binding
• GO:0008168 methyltransferase activity

Biological Process

• GO:0006370 7-methylguanosine mRNA capping
• GO:0032259 methylation
• GO:0106005 RNA 5'-cap (guanine-N7)-methylation

Cellular Component

• GO:0019028 viral capsid
• GO:0039624 viral outer capsid

External links

• PDB: RCSB:7yed, PDBe:7yed, PDBj:7yed
• PDBsum: 7yed
• PubMed: 36469786
• UniProt: C9E871