Structure of PDB 7mft Chain I

Receptor sequence

>7mftI (length=490) Species: 535026 (Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10)

KPTGFMEIKREKPAERDPLTRLKDWKEYSAPFSEEASKRQGARCMDCGTP
FCQIGADINGFTSGCPIYNLIPEWNGLVYRGRWKEALERLLKTNNFPEFT
GRVCPAPCEGSCTLAISDPAVSIKNIERTIIDKGFENGWIQPRIPKKRTG
KKVAIVGSGPAGLASADQLNQAGHSVTVFERADRAGGLLTYGIPNMKLEK
GIVERRIKLLTQEGIDFVTNTEIGVDITADELKEQFDAVILCTGAQKQRD
LLIEGRDSKGVHYAMDYLTLATKSYLDSNFKDKQFIDAKGKDVIVIGGGD
TGADCVATALRQKAKSVHQFGKHPKLPPARTNDNMWPEQPHVFTLEYAYE
EAEAKFGRDPREYSIQTTKMVADKNGKLKELHTIQMEKVKNEHGKYEFRE
LPGTEKVWPAQLVFIAIGFEGTEQPLLKQFGVNSVNNKISAAYGDYQTNI
DGVFAAGDARRGQSLIVWAINEGREVAREVDRYLMGSSVL

3D structure

• PDB: 7mft A counter-enzyme complex regulates glutamate metabolism in Bacillus subtilis.
• Chain: I
• Resolution: 3.9 Å

Enzymatic activity

• Enzyme Commision number: 1.4.1.13: glutamate synthase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	I	V105 G159 G161 P162 A163 E182 R183 G189 L190 K199 I225 T245 G246 M267 D302 F421 D460 S466 L467 I468	V103 G157 G159 P160 A161 E180 R181 G187 L188 K197 I223 T243 G244 M265 D300 F419 D458 S464 L465 I466
BS02	SF4	I	C46 C49 P52 C54 C114 T115 L116 V123	C44 C47 P50 C52 C112 T113 L114 V121
BS03	SF4	I	C67 N71 C106 A108 P109 C110 I125 K126 E129	C65 N69 C104 A106 P107 C108 I123 K124 E127

Gene Ontology

Molecular Function

• GO:0004355 glutamate synthase (NADPH) activity
• GO:0016491 oxidoreductase activity
• GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor
• GO:0051536 iron-sulfur cluster binding

Biological Process

• GO:0006537 glutamate biosynthetic process
• GO:0097054 L-glutamate biosynthetic process

External links

• PDB: RCSB:7mft, PDBe:7mft, PDBj:7mft
• PDBsum: 7mft
• PubMed: 34931064
• UniProt: O34399|GLTB_BACSU Glutamate synthase [NADPH] small chain (Gene Name=gltB)