Structure of PDB 6nue Chain I

Receptor sequence

>6nueI (length=295) Species: 1308 (Streptococcus thermophilus)

YKLYIMTFQNAHFGSGTLDSSKLTFSADRIFSALVLESLKMGKLDAFLAE
ANQDKFTLTDAFPFQFGPFLPKPIGYPKHDQIDQSVDVKEVRRQAKLSKK
LQFLALENVDDYLNGELFENEEHAVIDTVTKNQPHKDGNLYQVATTRFSN
DTSLYVIANESDLLNELMSSLQYSGLGGKRSSGFGRFELDIQNIPLELSD
RLTKNHSDKVMSLTTALPVDADLEEAMEDGHYLLTKSSGFAFSHATNENY
RKQDLYKFASGSTFSKTFEGQIVDVRPLDFPHAVLNYAKPLFFKL

3D structure

• PDB: 6nue Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.
• Chain: I
• Resolution: 3.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	I	G16 S17 G18 R31 S34 A35 V37 L38 L41 L46 T132 K133 N134 P136 H137 Y143 G180 K181 R182 S183 S240 F242 F244 R253 K254 H284 A285 L287	G14 S15 G16 R29 S32 A33 V35 L36 L39 L44 T130 K131 N132 P134 H135 Y141 G178 K179 R180 S181 S238 F240 F242 R251 K252 H282 A283 L285
BS02	ATP	I	R94 K98	R92 K96

Gene Ontology

Molecular Function

• GO:0003723 RNA binding

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:6nue, PDBe:6nue, PDBj:6nue
• PDBsum: 6nue
• PubMed: 30814678
• UniProt: A0A0A7HGA1|CSM4_STRTR CRISPR system Cms protein Csm4 (Gene Name=csm4)