Structure of PDB 4wqt Chain I

Receptor sequence

>4wqtI (length=1318) Species: 300852 (Thermus thermophilus HB8)

KKEVRKVRIALASPEKIRSWSYGEVEKPETINYRTLKPERDGLFDERIFG
PKSIVRRYRMGHIELATPAAHIWFVKDVPSKIGTLLDLSATELEQVLYFS
KYIVLDPKGAILNGVPVEKRQLLTDEEYRELRYGKQETYPLPPGVDALVK
DGEEVVKGQELAPGVVSRLDGVALYRFPRRVRVEYVVQPHMNVVVPEGAR
VEAGDKIVAAIDPEEEVIAEAEGVVHLHEPASILVVKARVYPFEDDVEVS
TGDRVAPGDVLADGGKVKSDVYGRVEVDLVRNVVRVVESYDIDARMGAEA
IQQLLKELDLEALEKELLEEMKHPSRARRAKARKRLEVVRAFLDSGNRPE
WMILEAVPVLPPDLRATSDLNDLYRRLINRNNRLKKLLAQGAPEIIIRNE
KRMLQEAVDALLDNGRRGAPVTNPGSDRPLRSLTDILSGKQGRFRQNLLG
KRVDYSGRSVIVVGPQLKLHQCGLPKRMALELFKPFLLKKMEEKGIAPNV
KAARRMLERQRDIKDEVWDALEEVIHGKVVLLNRAPTLHRLGIQAFQPVL
VEGQSIQLHPLVCEAFNADFDGDQMAVHVPLSSFAQAEARIQMLSAHNLL
SPASGEPLAKPSRDIILGLYYITQVRKEKKGAGLEFATPEEALAAHERGE
VALNAPIKVAGRETSVGRLKYVFANPDEALLAVAHGIVDLQDVVTVRYMG
KRLETSPGRILFARIVAEAVEDEKVAWELIQLDVPQEKNSLKDLVYQAFL
RLGMEKTARLLDALKYYGFTFSTTSGITIGIDDAVIPEEKKQYLEEADRK
LLQIEQAYEMGFLTDRERYDQILQLWTETTEKVTQAVFKNFEENYPFNPL
YVMAQSGARGNPQQIRQLCGLRGLMQKPSGETFEVPVRSSFREGLTVLEY
FISSHGARKGGADTALRTADSGYLTRKLVDVTHEIVVREADCGTTNYISV
PLFQPDEVTRSLRLRKRADIEAGLYGRVLAREVEVLGVRLEEGRYLSMDD
VHLLIKAAEAGEIQEVPVRSPLTCQTRYGVCQKCYGYDLSMARPVSIGEA
VGIVAAQSIGEPGTQLTMRTFTQGLPRVIELFEARRPKAKAVISEIDGVV
RIEETEEKLSVFVESEGFSKEYKLPKEARLLVKDGDYVEAGQPLTRGAID
PHQLLEAKGPEAVERYLVEEIQKVYRAQGVKLHDKHIEIVVRQMMKYVEV
TDPGDSRLLEGQVLEKWDVEALNERLIAEGKTPVAWKPLLMGVTKSALST
KSWLSAASFQNTTHVLTEAAIAGKKDELIGLKENVILGRLIPAGTGSDFV
RFTQVVDQKTLKAIEEAR

3D structure

• PDB: 4wqt The Ratcheted and Ratchetable Structural States of RNA Polymerase Underlie Multiple Transcriptional Functions.
• Chain: I
• Resolution: 4.4 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	I	C1194 C1201 C1204	C1024 C1031 C1034
BS02	MG	I	D739 D741 D743	D569 D571 D573

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:4wqt, PDBe:4wqt, PDBj:4wqt
• PDBsum: 4wqt
• PubMed: 25601758
• UniProt: Q8RQE8|RPOC_THET8 DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)