Structure of PDB 4k50 Chain I

Receptor sequence

>4k50I (length=460) Species: 31708 (rhinovirus A16)

GQIQISKHVKDVGLPSIHTPTKTKLQPSVFYDIFPGSKEPAVLTEKDPRL
KVDFDSALFSKYKGNTECSLNEHIQVAVAHYSAQLATLDIDPQPIAMEDS
VFGMDGLEALDLNTSAGYPYVTLGIKKKDLINNKTKDISKLKLALDKYDV
DLPMITFLKDELRKKDKIAAGKTRVIEASSINDTILFRTVYGNLFSKFHL
NPGVVTGCAVGCDPETFWSKIPLMLDGDCIMAFDYTNYDGSIHPIWFKAL
GMVLDNLSFNPTLINRLCNSKHIFKSTYYEVEGGVPSGCSGTSIFNSMIN
NIIIRTLVLDAYKHIDLDKLKIIAYGDDVIFSYKYKLDMEAIAKEGQKYG
LTITPADKSSEFKELDYGNVTFLKRGFRQDDKYKFLIHPTFPVEEIYESI
RWTKKPSQMQEHVLSLCHLMWHNGPEIYKDFETKIRSVSAGRALYIPPYE
LLRHEWYEKF

3D structure

• PDB: 4k50 Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts.
• Chain: I
• Resolution: 2.93 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	I	K63 K313 H314 Y335 K336	K63 K313 H314 Y335 K336
BS02	rna	I	H18 P20 K22 K24 E108 T114 S115 T122 K127 F157 K159 I176 A178 R188 H199 G211 C212 D213 G288 C289 Y325 E411 L414 H418	H18 P20 K22 K24 E108 T114 S115 T122 K127 F157 K159 I176 A178 R188 H199 G211 C212 D213 G288 C289 Y325 E411 L414 H418
BS03	rna	I	S293 Y325 D327 D328 F391 E395 S399 K404 K405 Q408 H412 S415	S293 Y325 D327 D328 F391 E395 S399 K404 K405 Q408 H412 S415

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:4k50, PDBe:4k50, PDBj:4k50
• PDBsum: 4k50
• PubMed: 23667424
• UniProt: Q82122|POLG_HRV16 Genome polyprotein