Structure of PDB 4k4i Chain I

Receptor sequence

>4k4iI (length=325) Species: 9606 (Homo sapiens)

TNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEA
CSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTA
QMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTV
QKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLD
SLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEF
ACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTPGRVLPTPT
EKDVFRLLGLPYREPAERDWLEHHH

3D structure

• PDB: 4k4i Structural basis for the binding and incorporation of nucleotide analogs with L-stereochemistry by human DNA polymerase lambda.
• Chain: I
• Resolution: 2.25 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D178 D180 D241
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	I	R538 R549	R289 R294
BS02	dna	I	W274 T370 T371 E465 E466 Y505 R514 R517 K521	W25 T121 T122 E216 E217 Y256 R265 R268 K272
BS03	dna	I	W342 G343 A344 G345 K347 T348 K472	W93 G94 A95 G96 K98 T99 K223
BS04	dna	I	W274 R275 G278 Y279 G304 G306 R308 M309 K312	W25 R26 G29 Y30 G55 G57 R59 M60 K63
BS05	1RY	I	R386 S417 R420 G426 Y505 S509 A510 N513 R517	R137 S168 R171 G177 Y256 S260 A261 N264 R268
BS06	CA	I	S339 I341 A344	S90 I92 A95
BS07	CA	I	D427 D429	D178 D180

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4k4i, PDBe:4k4i, PDBj:4k4i
• PDBsum: 4k4i
• PubMed: 25015085
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)