Structure of PDB 4k4h Chain I

Receptor sequence

>4k4hI (length=332) Species: 9606 (Homo sapiens)

ATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQE
ACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKT
AQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQT
VQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLL
DSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSE
FACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPG
RVLPTPTEKDVFRLLGLPYREPAERDWLEHHH

3D structure

• PDB: 4k4h Structural basis for the binding and incorporation of nucleotide analogs with L-stereochemistry by human DNA polymerase lambda.
• Chain: I
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D179 D181 D242
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	I	W274 T370 T371 E465 E466 Y505 R517 K521 E529	W26 T122 T123 E217 E218 Y257 R269 K273 E281
BS02	dna	I	W342 G343 A344 G345 K347 T348	W94 G95 A96 G97 K99 T100
BS03	dna	I	Y267 W274 R275 G278 Y279 G304 G306 R308 M309 K312	Y19 W26 R27 G30 Y31 G56 G58 R60 M61 K64
BS04	1RZ	I	R386 G416 S417 R420 G426 D427 Y505 N513 R517	R138 G168 S169 R172 G178 D179 Y257 N265 R269
BS05	CA	I	D427 D429	D179 D181
BS06	CA	I	D427 D429	D179 D181
BS07	CA	I	S509 A510	S261 A262
BS08	CA	I	S339 I341 A344	S91 I93 A96

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4k4h, PDBe:4k4h, PDBj:4k4h
• PDBsum: 4k4h
• PubMed: 25015085
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)