Structure of PDB 3hkz Chain I

Receptor sequence

>3hkzI (length=836) Species: 273057 (Saccharolobus solfataricus P2)

KNIKGIKFGILSPDEIRKMSVTAIITPMDPRLGVIEPGQKCPTCGNTLGN
CPGHFGHIELVRPVIHVGLVKHIYEFLKATCRRCGRVKISEDEIEKYSRI
YNAIKKRWPSAARRLTEYVKKTAMKAQVCPHCNEKQYKIKLTPSDIRERL
EKIPDSDVEILGYDPTTSRPEWMILTVLPVPPITIRPSEDDLTHKLVDIV
RINERLKESIDAGAPQLIIEDLWDLLQYHVATYFDNEIPGLPPSKHRSGR
PLRTLAQRLKGKEGRFRGNLSGKRVDFSSRTVISPDPNISIDEVGVPEII
AKTLTVPERITPWNIEKLRQFVINGPDKWPGANYVIRPDGRRIDLRYVKD
RKELASTLAPGYIIERHLIDGDIVLFNRQPSLHRISMMAHRVRVLKGLTF
RLNLLVCPPYNADFDGDEMNLHVPQSEEAIAEAKEIMLVHKNIITPRYGG
PIIGAAQDYISGAYLLTVKTTLLTKEEAQQILGVADVKIDLGEPAILAPR
EYYTGKQVISAFLPKDFNFHGQANVSSGPRLCKNEDCPHDSYVVIKNGIL
LEGVFDKKAIGNQQPESILHWLIKEYSDEYGKWLMDNLFRVFIRFVELQG
FTMRLEDVSLGDDVKKEIYNEIDRAKVEVDNLIQKYKNGELEPIPGRTLE
ESLENYILDTLDKLRSTAGDIASKYLDPFNFAYVMARTGARGSVLNITQM
AAMLGQQSVRGERIKRGYMTRTLPHFKPYDISPEARGFIYSSFRTGLKPT
ELFFHAAGGREGLVDTAVRTSQSGYMQRRLINALSDLRAEYDGTVRSLYG
EVVQVAYGDDGVFPMYSAHGKTVDVNRIFERVVGWK

3D structure

• PDB: 3hkz The X-ray crystal structure of RNA polymerase from Archaea.
• Chain: I
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	I	C61 H71	C44 H54
BS02	ZN	I	C98 Q144	C81 Q127
BS03	MG	I	D456 D458 D460	D413 D415 D417

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005737 cytoplasm

External links

• PDB: RCSB:3hkz, PDBe:3hkz, PDBj:3hkz
• PDBsum: 3hkz
• PubMed: 18235446
• UniProt: Q980R2|RPO1N_SACS2 DNA-directed RNA polymerase subunit Rpo1N (Gene Name=rpo1N)