Structure of PDB 3aoh Chain I

Receptor sequence

>3aohI (length=1289) Species: 300852 (Thermus thermophilus HB8)

KKEVRKVRIALASPEKIRSWSYGEVEKPETINYRTLKPERDGLFDERIFG
PIKDYECACGKYKRQRFEGKVCERCGVEVTKSIVRRYRMGHIELATPAAH
IWFVKDVPSKIGTLLDLSATELEQVLYFSKYIVLDPKGAILNGVPVEKRQ
LLTDEEYRELRYGKQETYPLPPGVDALVKDGEEVVKGQELAPGVVSRLDG
VALYRLVVKARVYPFEDDVEVSTGDRVAPGDVLADGGKVKSDVYGRVEVD
LVRNVVRVVESYDIDARMGAEAIQQLLKELDLEALEKELLEEMKHPSRAR
RAKARKRLEVVRAFLDSGNRPEWMILEAVPVLPPDLRPSDLNDLYRRLIN
RNNRLKKLLAQGAPEIIIRNEKRMLQEAVDALLDNGRRGAPVTNPGSDRP
LRSLTDILSGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKRMA
LELFKPFLLKKMEEKGIAPNVKAARRMLERQRDIKDEVWDALEEVIHGKV
VLLNRAPTLHRLGIQAFQPVLVEGQSIQLHPLVCEAFNADFDGDQMAVHV
PLSSFAQAEARIQMLSAHNLLSPASGEPLAKPSRDIILGLYYITQVRKEK
KGAGLEFATPEEALAAHERGEVALNAPIKVAGRETSVGRLKYVFANPDEA
LLAVAHGIVDLQDVVTVRYMGKRLETSPGRILFARIVAEAVEDEKVAWEL
IQLDVPQEKNSLKDLVYQAFLRLGMEKTARLLDALKYYGFTFSTTSGITI
GIDDAVIPEEKKQYLEEADRKLLQIEQAYEMGFLTDRERYDQILQLWTET
TEKVTQAVFKNFEENYPFNPLYVMAQSGARGNPQQIRQLCGLRGLMQKPS
GETFEVPVRSSFREGLTVLEYFISSHGARKGGADTALRTADSGYLTRKLV
DVTHEIVVREADCGTTNYISVPLFQPDEVTRSLRLRKRADIEAGLYGRVL
AREVEVLGVRLEEGRYLSMDDVHLLIKAAEAGEIQEVPVRSPLTCQTRYG
VCQKCYGYDLSMARPVSIGEAVGIVAAQSIGEPGTQLTDITQGLPRVIEL
FEARRPKAKAVISEIDGVVRIEETEEKLSVFVESEGFSKEYKLPKEARLL
VKDGDYVEAGQPLTRGAIDPHQLLEAKGPEAVERYLVEEIQKVYRAQGVK
LHDKHIEIVVRQMMKYVEVTDPGDSRLLEGQVLEKWDVEALNERLIAEGK
TPVAWKPLLMGVTKSALSTKSWLSAASFQNTTHVLTEAAIAGKKDELIGL
KENVILGRLIPAGTGSDFVRFTQVVDQKTLKAIEEARKE

3D structure

• PDB: 3aoh Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein
• Chain: I
• Resolution: 4.1 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	I	C1194 C1204	C995 C1005
BS02	MG	I	D739 D741 D743	D540 D542 D544

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:3aoh, PDBe:3aoh, PDBj:3aoh
• PDBsum: 3aoh
• PubMed: 21124318
• UniProt: Q8RQE8|RPOC_THET8 DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)