Structure of PDB 8g9u Chain H

Receptor sequence

>8g9uH (length=582) Species: 486 (Neisseria lactamica)

MILHALTQYYQRKAESDGGIAQEGFENKEIPFIIVIDKQGNFIQLEDTRE
LKVKKKVGRTFLVPKGLGRSGSKSYEVSNLLWDHYGYVLAYAGEKGQEQA
DKQHASFTAKVNELKQALPDDAGVTAVAAFLSSAEEKSKVMQAANWAECA
KVKGCNLSFRLVDEAVDLVCQSKAVREYVSQANQTQSDNAQKGICLVTGK
AAPIARLHNAVKGVNAKPAPFASVNLSAFESYGKEQGFAFPIGEQAMFEY
TTALNTLLAGENRFRIGDVTTVCWGAKRTPLEESLASMINGGGKDNPDAH
IDAVKALYKSLYNGQYCKPDGEDKFYLLGLSPNSARIVVRFWHETTVAAL
SESIAAWYDDLQMVRGENSPYPEYMPLPRLLGNLVLDGKMENLPSDLIAQ
ITDAALNNRVLPVSLLQAALRRNKAEQKITYGRASLLKAYINRAIRAGRL
KNMKELTMGLDRNRQDIGYVLGRLFAVLEKIQAEANPGLNATIADRYFGS
ASSTPIAVFGTLMRLLPHHLNKLEFEGRAVQLQWEIRQILEHCQRFPNHL
NLEQQGLFAIGYYHETQFLFTKDALKNLFNEA

3D structure

• PDB: 8g9u Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications.
• Chain: H
• Resolution: 3.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	H	A228 S231 Y232	A228 S231 Y232
BS02	dna	H	S70 G71 S72 K73 R206 L207 K217 P220 S223 N225 Q236 S334 A335 R336 L386 L393 R422 A425 Q427 Q482 N486 L489 N490 A491 R496 L515 H518 H519 K522	S70 G71 S72 K73 R206 L207 K217 P220 S223 N225 Q236 S334 A335 R336 L386 L393 R422 A425 Q427 Q482 N486 L489 N490 A491 R496 L515 H518 H519 K522
BS03	dna	H	K52 K54 R69 S70 G71 S74 K95 Q99 K153 G154 K217 P218 R263 R265 Y312 N313 P376 P378 R379	K52 K54 R69 S70 G71 S74 K95 Q99 K153 G154 K217 P218 R263 R265 Y312 N313 P376 P378 R379
BS04	dna	H	N368 P370 Y371 K480 E484 F525 E526 G527 R528 V530 Q531 R537 Q567 F570 T571 K572 F579	N368 P370 Y371 K480 E484 F525 E526 G527 R528 V530 Q531 R537 Q567 F570 T571 K572 F579

External links

• PDB: RCSB:8g9u, PDBe:8g9u, PDBj:8g9u
• PDBsum: 8g9u
• PubMed: 38242128
• UniProt: D0W8X5