Structure of PDB 8a98 Chain H

Receptor sequence

>8a98H (length=221) Species: 347515 (Leishmania major strain Friedlin)

AFPSRKDAFRAQRKGAKKHRPEIIVIDLKDHVLGRAAAVVAKQLLLGKKI
TVVRCEQLNIAGTEIRNKIKYLQYLRKRKLTNPTKGPFHHRAPSDVFVRT
VRSMLPRYTKRGMKALNSLVAYEGIPPNVVRTGGRVVIPRAQRHVCYRSE
RPYTVLGNMCKHVGWKYSDVVANLEKARVEKASRHHEKQAKLRDAWKSAR
KEALAKMPKHNVEVLKKFGYA

3D structure

• PDB: 8a98 CRYO-EM STRUCTURE OF LEISHMANIA MAJOR 80S RIBOSOME : snoRNA MUTANT
• Chain: H
• Resolution: 2.46 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	H	G35 R36 K43 I66 R67 I70 Q74 R77 K78 R79 K80 L81 S104 M105 K111 R141 V146 C147 Y148 R149 R152	G34 R35 K42 I65 R66 I69 Q73 R76 K77 R78 K79 L80 S103 M104 K110 R140 V145 C146 Y147 R148 R151
BS02	rna	H	A2 R11 H20 I24 K50 I51 N83 K86 G87 P88 F89 R108 Y109 S119 Y123 E124 N129 R132 R185 H186 K189	A1 R10 H19 I23 K49 I50 N82 K85 G86 P87 F88 R107 Y108 S118 Y122 E123 N128 R131 R184 H185 K188
BS03	rna	H	A2 F3 P4 S5 R6 K7 F10 R79 H90 R92 K167 Y168	A1 F2 P3 S4 R5 K6 F9 R78 H89 R91 K166 Y167
BS04	rna	H	R11 R14 V131 R132 G134 G135 R136 H145 H187 W197 R201 H211 G220	R10 R13 V130 R131 G133 G134 R135 H144 H186 W196 R200 H210 G219

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8a98, PDBe:8a98, PDBj:8a98
• PDBsum: 8a98
• PubMed: 38722744
• UniProt: Q4QFG2