Structure of PDB 7w4o Chain H

Receptor sequence

>7w4oH (length=1411) Species: 10036 (Mesocricetus auratus)

PLAFCGTENHSAAYRVDQGVLNNGCFVDALNVVPHVFLLFITFPILFIGW
GSQSSKVHIHHSTWLHFPGHNLRWILTFILLFVLVCEIAEGILSDGVTES
RHLHLYMPAGMAFMAAITSVVYYHNIETSNFPKLLIALLIYWTLAFITKT
IKFVKFYDHAIGFSQLRFCLTGLLVILYGMLLLVEVNVIRVRRYIFFKTP
REVKPPEDLQDLGVRFLQPFVNLLSKGTYWWMNAFIKTAHKKPIDLRAIG
KLPIAMRALTNYQRLCVAFDAQARPQGARAIWRALCHAFGRRLILSSTFR
ILADLLGFAGPLCIFGIVDHLGKKTQFLGVYFVSSQEFLGNAYVLAVLLF
LALLLQRTFLQASYYVAIETGINLRGAIQTKIYNKIMHLSTSNLSMGEMT
AGQICNLVAIDTNQLMWFFFLCPNLWAMPVQIIVGVILLYYILGVSALIG
AAVIILLAPVQYFVATKLSQAQRSTLEHSNERLKQTNEMLRGMKLLKLYA
WESIFCSRVEVTRRKEMTSLRAFAVYTSISIFMNTAIPIAAVLITFVGHV
SFFKESDLSPSVAFASLSLFHILVTPLFLLSSVVRSTVKALVSVQKLSEF
LSSAEIREDNFCVQIIGGFFTWTPDGIPTLSNITIRIPRGQLTMIVGQVG
CGKSSLLLATLGEMQKVSGAVFWNSSRGPVAYASQKPWLLNATVEENITF
ESPFNKQRYKMVIEACSLQPDIDILPHGDQTQIGERGINLSGGQRQRISV
ARALYQQTNVVFLDDPFSALDVHLSDHLMQAGILELLRDDKRTVVLVTHK
LQYLPHADWIIAMKDGTIQREGTLKDFQRSECQLFEHWKTLMNELEKERA
CTKYLSSAGILLLSLLVFSQLLKHMVLVAIDYWLAKWTDSDQSVYAMVFT
LLCSLGIVLCLVTSVTVEWTGLKVAKRLHRSLLNRIILAPMRFFETTPLG
SILNRFSSDCNTIDQHIPSTLECLSRSTLLCVSALTVISYVTPVFLVALL
PLAVVCYFIQKYFRVASRDLQQLDDTTQLPLLSHFAETVEGLTTIRAFRY
EARFQQKLLEYTDSNNIASLFLTAANRWLEVRMEYIGACVVLIAAATSIS
NSLHRELSAGLVGLGLTYALMVSNYLNWMVRNLADMEIQLGAVKRIHALL
KTEAESYEGLLAPSLIPKNWPDQGKIQIQNLSVRYDSSLKPVLKHVNALI
SPGQKIGICGRTGSGKSSFSLAFFRMVDMFEGRIIIDGIDIAKLPLHTLR
SRLSIILQDPVLFSGTIRFNLDPEKKCSDSTLWEALEIAQLKLVVKALPG
GLDAIITEGGENFSQGQRQLFCLARAFVRKTSIFIMDEATASIDMATENI
LQKVVMTAFADRTVVTIAHRVHTILSADLVMVLKRGAILEFDKPETLLSQ
KDSVFASFVRA

3D structure

• PDB: 7w4o Structural insights into the mechanism of pancreatic K ATP channel regulation by nucleotides.
• Chain: H
• Resolution: 2.96 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	H	R1111 E1114 Y1354 V1361 T1381 G1382 G1384 K1385 S1386 S1387	R942 E945 Y1185 V1192 T1212 G1213 G1215 K1216 S1217 S1218
BS02	MG	H	S720 Q775 D854	S654 Q685 D764
BS03	E2H	H	P551 I552 V555 H584 D1031 T1286 M1290	P538 I539 V542 H571 D881 T1117 M1121
BS04	ATP	H	S408 W688 V715 G716 G718 K719 S720 S721 Q775 H889 E1480 N1481 S1483 G1485	S395 W622 V649 G650 G652 K653 S654 S655 Q685 H799 E1311 N1312 S1314 G1316

Gene Ontology

Molecular Function

• GO:0005267 potassium channel activity
• GO:0005524 ATP binding
• GO:0008281 sulfonylurea receptor activity
• GO:0016887 ATP hydrolysis activity
• GO:0019829 ATPase-coupled monoatomic cation transmembrane transporter activity
• GO:0042626 ATPase-coupled transmembrane transporter activity
• GO:0043531 ADP binding
• GO:0044325 transmembrane transporter binding
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0001508 action potential
• GO:0006813 potassium ion transport
• GO:0031669 cellular response to nutrient levels
• GO:0035774 positive regulation of insulin secretion involved in cellular response to glucose stimulus
• GO:0046676 negative regulation of insulin secretion
• GO:0050905 neuromuscular process
• GO:0055085 transmembrane transport
• GO:0061535 glutamate secretion, neurotransmission
• GO:0071805 potassium ion transmembrane transport
• GO:0098655 monoatomic cation transmembrane transport
• GO:0098662 inorganic cation transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0008282 inward rectifying potassium channel
• GO:0016020 membrane
• GO:0032991 protein-containing complex
• GO:0098793 presynapse

External links

• PDB: RCSB:7w4o, PDBe:7w4o, PDBj:7w4o
• PDBsum: 7w4o
• PubMed: 35589716
• UniProt: A0A1U7R319