Structure of PDB 7u3b Chain H

Receptor sequence

>7u3bH (length=696) Species: 54571 (Streptomyces venezuelae)

MQVWPGQAYPLGATYDGAGTNFAVFSEAAHRIELCLLHDDGSETAVELRE
TDAFVRHAYLPGVMPGQRYGFRVHGPYAPERGLRCNAAKLLLDPYARAVS
GRVRWGEAVYGYPFGRPDARNDLDSAPDTMTSVVVNPYFDWGDDRRPRTE
YHHTVIYEAHVKGLTMLHPDLPEELRGTYAGLAHPSVIGHLRELGVTALE
LMPVHQFVNDHRLVDAGLSNYWGYNTIGFFAPHNAYASWGDRGQQVLEFK
SAVRALHQAGIEVILDVVYNHTAEGNHLGPTLSMRGLDNPSYYRLADDPR
YYMDTTGTGNSLLMRSPHVLQLIMDSLRYWVTEMHVDGFRFDLAATLARQ
FHEVDRLSSFFDLVQQDPVVSQVKLIAEPWDVGEGGYQVGNFPPLWTEWN
GKYRDCVRDLWRGEPRTLAEFASRLTGSSDLYQDDGRRPLASVNFVTCHD
GFTLRDLVSYNEKRNEANGEGNRDGENYNRSWNCGEEGETEDVGITELRA
RQMRNFLATLMLSQGVPMLSHGDEFGRTQGGNNNAYCQDNEVSWVRWPKE
AEATLLRFTRSMVRLRREHPVFRRRRFFHGRPVELTDIAWFTPEGEEMTS
RDWQAAHAQALTVFLNGNAISEPGTQGERIADDSFLLMFNASAKELEFVV
PHGRYWRMVVDTSDPEGMPPQQGPELAGGERVTLAPLSLTVLRRPA

3D structure

• PDB: 7u3b Allosteric regulation of glycogen breakdown by the second messenger cyclic di-GMP.
• Chain: H
• Resolution: 3.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	C2E	H	Q433 R438 F580	Q433 R438 F577
BS02	A16	H	R212 W222 Y224 D342 L343 H449 D450 E470 N534	R212 W222 Y224 D342 L343 H449 D450 E470 N534
BS03	C2E	H	H30 R31 E47 R49 E50 T51 R56	H30 R31 E47 R49 E50 T51 R56

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004133 glycogen debranching enzyme activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005980 glycogen catabolic process

External links

• PDB: RCSB:7u3b, PDBe:7u3b, PDBj:7u3b
• PDBsum: 7u3b
• PubMed: 36192422
• UniProt: A0A5P2ALW6