Structure of PDB 7rmv Chain H

Receptor sequence

>7rmvH (length=553) Species: 4932 (Saccharomyces cerevisiae)

MKYVVVSGGVISGIGKGVLASSTGMLMKTLGLKVTSIKIDPYMNIDAGTM
SPLEHGECFVLDDGGETDLDLGNYERYLGVTLTKDHNITTGKIYSHVIAK
ERKGDYLGKTVQIVPHLTNAIQDWIERVAKIPVDDTGMEPDVCIIELGGT
VGDIESAPFVEALRQFQFKVGKENFALIHVSLVPVIHGEQKTKPTQAAIK
GLRSLGLVPDMIACRCSETLDKPTIDKIAMFCHVGPEQVVNVHDVNSTYH
VPLLLLEQKMIDYLHARLKLDEISLTEEEELLSKWKATTGNFDETVKIAL
VGKYTNLKDSYLSVIKALEHSSMKCRRKLDIKWVEATDLEPEAQESNKTK
FREAWNMVSTADGILIPGGFGVRGTEGMVLAARWARENHIPFLGVCLGLQ
IATIEFTRSVLGRKDSHSAEFYPDIDEKNHVVVFMMRLGLRPTFFQNETE
WSQIKKLYGDVSEVHERHRHRYEINPKMVDELENNGLIFVGKDDTGKRCE
ILELKNHPYYIATQYHPEYTSKVLDPSKPFLGLVAASAGILQDVIEGKYD
LEA

3D structure

• PDB: 7rmv Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
• Chain: H
• Resolution: 6.7 Å

Enzymatic activity

• Enzyme Commision number: 6.3.4.2: CTP synthase (glutamine hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	H	G13 G15 K16 G17 V18 R215 V242	G13 G15 K16 G17 V18 R215 V242
BS02	UTP	H	S12 P41 E155	S12 P41 E155
BS03	UTP	H	Q190 K191 T192 K193	Q190 K191 T192 K193

Gene Ontology

Molecular Function

• GO:0003883 CTP synthase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006221 pyrimidine nucleotide biosynthetic process
• GO:0006241 CTP biosynthetic process
• GO:0006541 glutamine metabolic process
• GO:0008654 phospholipid biosynthetic process
• GO:0019856 pyrimidine nucleobase biosynthetic process
• GO:0044210 'de novo' CTP biosynthetic process

Cellular Component

• GO:0000324 fungal-type vacuole
• GO:0005737 cytoplasm
• GO:0097268 cytoophidium

External links

• PDB: RCSB:7rmv, PDBe:7rmv, PDBj:7rmv
• PDBsum: 7rmv
• PubMed: 34734801
• UniProt: P28274|URA7_YEAST CTP synthase 1 (Gene Name=URA7)