Structure of PDB 7miu Chain H

Receptor sequence

>7miuH (length=542) Species: 10090 (Mus musculus)

MKYILVTGGVISGIGKGIIASSIGTILKSCGLRVTAIKIDPYINIDAGTF
SPYEHGEVFVLNDGGEVDLDLGNYERFLDINLYKDNNITTGKIYQHVINK
ERRGDYLGKTVQVVPHITDAIQEWVMNQAKVSVDGNKEDPQICVIELGGT
IGDIEGMAFVEAFRQFQFKAKKENFYNIHVSLVPQPSATGEQKTKPTQNS
VRALRGLGLSPDLIVCRSSTPIEMAVKEKISMFCHVNPEQVICIHDVSSI
YRVPLLLEEQGVVKYFQERLGLPLLFKWKAMADRYERLQKICSIALVGKY
TKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEPVTKAEDPVKFHEA
WQKLCLADGILVPGGFGIRGTLGKLQAISWARTKKIPFLGICLGMQLAVI
EFARNCLNLKDANSTEFEPNTPVPLVIDMPETMRLGLRRTVFTTENSILK
KLYGDVPYIEERHRHRYEVNPNLINQFENKDLCFVGEDVDGKRMEIVELT
SHPYFIGVQFHPEFSSRPMKPSPPYLGLLLAATGNLNAHLQQ

3D structure

• PDB: 7miu Structural basis for isoform-specific inhibition of human CTPS1.
• Chain: H
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 6.3.4.2: CTP synthase (glutamine hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLN	H	G371 G372 F373 C399 L400 Q403 E423 H480 R481 Y482 H526	G364 G365 F366 C392 L393 Q396 E416 H465 R466 Y467 H511
BS02	ZG4	H	I19 R76 F77 R217 D246 V247 I250 Y251 V253 W285 F318 K319 E322 H323	I19 R76 F77 R217 D246 V247 I250 Y251 V253 W278 F311 K312 E315 H316
BS03	UTP	H	E191 K193 T194 K195 Q198 K229	E191 K193 T194 K195 Q198 K229
BS04	UTP	H	K38 D40 H55 G148 G149 D153	K38 D40 H55 G148 G149 D153

Gene Ontology

Molecular Function

• GO:0003883 CTP synthase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0016879 ligase activity, forming carbon-nitrogen bonds
• GO:0042802 identical protein binding

Biological Process

• GO:0006221 pyrimidine nucleotide biosynthetic process
• GO:0006241 CTP biosynthetic process
• GO:0006541 glutamine metabolic process
• GO:0044210 'de novo' CTP biosynthetic process

Cellular Component

• GO:0005739 mitochondrion

External links

• PDB: RCSB:7miu, PDBe:7miu, PDBj:7miu
• PDBsum: 7miu
• PubMed: 34583994
• UniProt: P70303|PYRG2_MOUSE CTP synthase 2 (Gene Name=Ctps2)