Structure of PDB 6vz8 Chain H

Receptor sequence

>6vz8H (length=531) Species: 3702 (Arabidopsis thaliana)

FISRFAPDQPRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSSSI
RNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSGLADALLDS
VPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRIIE
EAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPGYMSRMPKPPED
SHLEQIVKPVLYVGGGCLNDELGRFVELTGIPVASTLMGLGSYPCDDELS
LHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRVTGIVHIDIDSAEIGKNK
TPHVSVCGDVKLALQGMNKVLENRAEELKLDFGWRNELNVQKQKFPLSFG
EAIPPQYAIKVLDELTDGKAIISTGVGQHQMWAAQFYNYKKPRQWLSSGG
LGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVENLPVK
VLLLNNQHLGMVMQWEDRFYKANRAHTFLGDPAQIFPNMLLFAAACGIPA
ARVTKKADLREAIQTMLDTPGPYLLDVICPH

3D structure

• PDB: 6vz8 Structures of fungal and plant acetohydroxyacid synthases.
• Chain: H
• Resolution: 3.45 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y118 G120 G121 A122 S123 E144 T167 F206 Q207 E208 K256 M351 V378 V485 G511 M513 D538 N565 H567 L568 M570 V571 W574
• Catalytic site (residue number reindexed from 1): Y32 G34 G35 A36 S37 E58 T81 F120 Q121 E122 K170 M256 V283 V376 G402 M404 D429 N456 H458 L459 M461 V462 W465
• Enzyme Commision number: 2.2.1.6: acetolactate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	H	H567 L568 G569	H458 L459 G460
BS02	FAD	H	G307 G308 G309 T331 L332 L349 H352 R373 R377 V378 I396 G508	G214 G215 G216 T236 L237 L254 H257 R278 R282 V283 I291 G399
BS03	TPP	H	V485 G486 Q487 H488 G511 M513 G537 D538 G539 S540 L568 G569 M570 V571	V376 G377 Q378 H379 G402 M404 G428 D429 G430 S431 L459 G460 M461 V462
BS04	TPP	H	E144 P170	E58 P84

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0003984 acetolactate synthase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0009082 branched-chain amino acid biosynthetic process

External links

• PDB: RCSB:6vz8, PDBe:6vz8, PDBj:6vz8
• PDBsum: 6vz8
• PubMed: 32640464
• UniProt: P17597|ILVB_ARATH Acetolactate synthase, chloroplastic (Gene Name=ALS)