Structure of PDB 5utu Chain H

Receptor sequence

>5utuH (length=487) Species: 353152 (Cryptosporidium parvum Iowa II) [Search protein sequence]

KMESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITGS
LHLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFAW
KNETIEDYWVCLNDAMTWRICGPNLIVDDGGDATLILHEGVKAEIEYEKY
NKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGMLKDLYGV
SEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCRQSLLHGL
FNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDPICALQAS
MEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYIANIGHFD
DEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRLVNLGCAT
GHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSKELDEYVA
RLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

PDB

5utu 2.65 Angstrom Resolution Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with SAH and NAD

Chain

Resolution

2.65 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
Catalytic site (residue number reindexed from 1)	H52 S75 S80 D129 E203 N228 K233 D237 N238 C242 H348 H402 S410 Q414
Enzyme Commision number	3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	NAD	H	K489 Y493	K481 Y485
BS02	NAD	H	T212 T214 N246 C250 G277 E278 V279 E298 I299 T331 N333 I354 G355 N403 H410	T204 T206 N238 C242 G269 E270 V271 E290 I291 T323 N325 I346 G347 N395 H402
BS03	SAH	H	L52 H53 T55 N78 D137 E211 T212 K241 D245 H356 F357 L401 H410 M415	L51 H52 T54 N77 D129 E203 T204 K233 D237 H348 F349 L393 H402 M407
BS04	ADN	H	H53 T55 E57 D137 E211 K241 D245 G409 H410 M415	H52 T54 E56 D129 E203 K233 D237 G401 H402 M407

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0004013	adenosylhomocysteinase activity
GO:0016787	hydrolase activity

	Biological Process
GO:0006730	one-carbon metabolic process
GO:0033353	S-adenosylmethionine cycle

	Cellular Component
GO:0005829	cytosol

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:5utu, PDBe:5utu, PDBj:5utu
PDBsum	5utu
PubMed
UniProt	Q5CPH1

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