Structure of PDB 5sc9 Chain H

Receptor sequence

>5sc9H (length=421) Species: 9606 (Homo sapiens)

GTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSV
ERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFSPLSYRPVAI
ALDTKGPGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGP
EGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQ
KMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIM
LSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTE
VTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAA
RQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVI
VVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 5sc9 Anthraquinone derivatives as ADP-competitive inhibitors of liver pyruvate kinase.
• Chain: H
• Resolution: 1.685 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	H	T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 G532 Y533 T534	T322 T323 T324 S327 W372 R379 G404 R406 P407 G408 S409 G410 Y411 T412
BS02	OXL	H	K282 E284 A305 G307 D308 T340	K160 E162 A183 G185 D186 T218
BS03	I7K	H	T62 R85 N87 H90 G307 S374 G375 A378 K379	T42 R65 N67 H70 G185 S252 G253 A256 K257
BS04	MG	H	E284 D308	E162 D186

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:5sc9, PDBe:5sc9, PDBj:5sc9
• PDBsum: 5sc9
• PubMed: 35290845
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)