Structure of PDB 5ey8 Chain H

Receptor sequence

>5ey8H (length=595) Species: 1772 (Mycolicibacterium smegmatis)

DGQIKFSIVAHVERWAKVRGDKAYRFLDFSTERDGVPRDLTWAQFSARNR
AVAARLQQVTQPGDRVAILCPQNLDYLVAFFGALYAGRIAVPLFDPSEHV
GRLHAVLDNCHPSAILTTTEAAEGVRKFFRTRPANQRPRVIAVDAVPDDV
ASTWVNPDEPDETTIAYLQYTSGRIPTGVQITHLNLATNVVQVIEALEGE
EGDRGLSWLPFFHDMGLITALLMIGHYFTFMTPAAFVRRPERWIRELARK
EGDTTISVAPNFAFDHAAARGVPKPGSPPLDLSNVKAVLNGSEPISAATV
RRFNEAFGPFGFPPKAIKPSYGLAEATLFVSTTPSAEEPKIITVDRDQLN
SGRIVEVDADSPKAVAQASAGKVGIAEWAVIVDAESATELPDGQVGEIWI
SGQNMGTGYWGKPEESVATFQNILKSRTNPSHAEGATDDATWVRTGDYGA
FYDGDLYITGRVKDLVIIDGRNHYPQDLEYSAQEASKAIRTGYVAAFSVP
ANQLPDEVFENAHSGIKRDPDDTSEQLVIVAERAAHKLDIGPITDDIRAA
IAVRHGVTVRDVLLTAAGAIPRTSSGKIGRRACRAAYLDGSLRAG

3D structure

• PDB: 5ey8 Insight into Structure-Function Relationships and Inhibition of the Fatty Acyl-AMP Ligase (FadD32) Orthologs from Mycobacteria.
• Chain: H
• Resolution: 3.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N207 H231 A346
• Catalytic site (residue number reindexed from 1): N189 H213 A324
• Enzyme Commision number: 6.2.1.20: long-chain-fatty-acid--[acyl-carrier-protein] ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	5SV	H	H231 D232 S314 S342 Y343 G344 L345 D469 I480	H213 D214 S292 S320 Y321 G322 L323 D447 I458

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008922 long-chain fatty acid [acyl-carrier-protein] ligase activity
• GO:0016874 ligase activity
• GO:0070566 adenylyltransferase activity

Biological Process

• GO:0006631 fatty acid metabolic process
• GO:0006633 fatty acid biosynthetic process
• GO:0008610 lipid biosynthetic process

Cellular Component

• GO:0005886 plasma membrane

External links

• PDB: RCSB:5ey8, PDBe:5ey8, PDBj:5ey8
• PDBsum: 5ey8
• PubMed: 26900152
• UniProt: A0R618|FAA32_MYCS2 Long-chain-fatty-acid--AMP ligase FadD32 (Gene Name=fadD32)