Structure of PDB 5ew1 Chain H

Receptor sequence

>5ew1H (length=251) Species: 9606 (Homo sapiens)

IVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPW
DKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDI
ALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETGQP
SVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGG
PFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQF
G

3D structure

• PDB: 5ew1 Through-bond effects in the ternary complexes of thrombin sandwiched by two DNA aptamers.
• Chain: H
• Resolution: 2.95 Å

Enzymatic activity

• Enzyme Commision number: 3.4.21.5: thrombin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	H	E23 I24 G25 M26 P28 W29 D116 H119 P120 C122 Y134 K135 G136 R137 N159 M201 K202 R206 W207	E8 I9 G10 M11 P13 W14 D113 H116 P117 C119 Y134 K135 G136 R137 N157 M204 K205 R211 W212
BS02	dna	H	Y89 H91 P92 R93 Y94 N95 W96 R97 R101 R126 L130 A132 I162 V163 R165 K169 D178 F181 H230 R233 W237 K240 F245	Y85 H87 P88 R89 Y90 N91 W92 R93 R98 R123 L130 A132 I160 V161 R163 K167 D176 F179 H235 R238 W242 K245 F250
BS03	dna	H	I24 R75 Y76 R77A I79 Y117	I9 R70 Y71 R73 I75 Y114
BS04	0G6	H	H57 L99 I174 D189 A190 S195 S214 W215 G216	H43 L96 I172 D192 A193 S198 S219 W220 G221

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0005509 calcium ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0007596 blood coagulation

External links

• PDB: RCSB:5ew1, PDBe:5ew1, PDBj:5ew1
• PDBsum: 5ew1
• PubMed: 27899589
• UniProt: P00734|THRB_HUMAN Prothrombin (Gene Name=F2)