Structure of PDB 4k2s Chain H

Receptor sequence

>4k2sH (length=396) Species: 290398 (Chromohalobacter israelensis DSM 3043)

SLKIRDAYTIVTCPGRNFVTLKIVTESGTHGIGDATLNGREMAVAAYLDE
HVVPALIGRDAGRIEDTWQYLYRGAYWRRGPVTMTAIAAVDMALWDIKAK
AAGMPLYQLLGGKSRERVMTYAHCTGQTIEDCLGEVARHVELGYRAVRVQ
SGVPGIETTYGVEPADSSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLH
VLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCVPAENQESLRLIREHTTT
PLAIGEVFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVADLASLYH
VRTGFHGATDLSPVCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYR
FEDGHFLAGESPGHGVDIDEELAAKYPYERASLPVNRLEDGTLWHW

3D structure

• PDB: 4k2s Crystal structure of the mutant P317A of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and d-gluconate
• Chain: H
• Resolution: 1.699 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H124 R149 Q151 Y161 D213 H215 E239 G264 E265 R286 P288 H315 G316 E342 W405
• Catalytic site (residue number reindexed from 1): H123 R148 Q150 Y160 D204 H206 E230 G255 E256 R277 P279 H306 G307 E333 W396
• Enzyme Commision number: 4.2.1.-
  4.2.1.39: gluconate dehydratase.
  4.2.1.8: mannonate dehydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GCO	H	N39 Y161 D213 H215 E265 H315 D319 E342	N38 Y160 D204 H206 E256 H306 D310 E333
BS02	MG	H	D213 E239 E265	D204 E230 E256

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0008927 mannonate dehydratase activity
• GO:0016829 lyase activity
• GO:0046872 metal ion binding
• GO:0047929 gluconate dehydratase activity

Biological Process

• GO:0009063 amino acid catabolic process
• GO:0016052 carbohydrate catabolic process

External links

• PDB: RCSB:4k2s, PDBe:4k2s, PDBj:4k2s
• PDBsum: 4k2s
• UniProt: Q1QT89|DMGD_CHRSD D-galactonate dehydratase family member ManD (Gene Name=manD)