Structure of PDB 3wfr Chain H

Receptor sequence

>3wfrH (length=463) Species: 32630 (synthetic construct)

AAALNFYLSYFDDVAKVLPREHYCFIVGGWVRDRILGEPVGYNIDVDFLT
TADPVELAKNFAKRIGGHFFVFEKRGFLIKRPTIASVVLHLPPYRYRFDF
SPLKGKDLEKALIEDLKERDFTANAIAVNLDDVLSTIVYDPTGGIKDLEQ
GLLRPVSIENLKRDPVRVLRGFRIAIEKNLQLTEDFYEFVKEDPRIVLKS
AVERITHELFKIMKEKTAHKVIRELYEYGVLEAIIPEIGRLREVKDPLDE
HTLKTLEYLEQVIEDRAKYLSAELLENFGKKRVLGEFTDVELLKWGALFH
DIGKPQTFAFYEHDKVGAQIVREIGERLRWGDEATEFVAKLVRHHLRPFF
LREAFKKGELKRRGMANFWRECGDIAPHLFLLSIADAMASGDEEEDIKAL
METIAELESFNRNEMKEEIQKAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAA

3D structure

• PDB: 3wfr Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase
• Chain: H
• Resolution: 3.501 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	H	D60 F83 F85 K87 I97 D112 R132 D133 N137 D182 R185 R188 E221 R222 L309 R361 G363 D364 X471 X474 X477	D47 F70 F72 K74 I84 D99 R119 D120 N124 D164 R167 R170 E203 R204 L284 R329 G331 D332 X431 X434 X437
BS02	CTP	H	G42 R45 D58 D60 R132 D133 N137 D182 R185 R188	G29 R32 D45 D47 R119 D120 N124 D164 R167 R170
BS03	POP	H	G42 R45 R188	G29 R32 R170
BS04	MG	H	D58 D60	D45 D47

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0016779 nucleotidyltransferase activity

Biological Process

• GO:0006396 RNA processing

External links

• PDB: RCSB:3wfr, PDBe:3wfr, PDBj:3wfr
• PDBsum: 3wfr
• PubMed: 24389024
• UniProt: O67911|CATNT_AQUAE CC-adding tRNA nucleotidyltransferase (Gene Name=aq_2158)