Structure of PDB 3sbr Chain H

Receptor sequence

>3sbrH (length=581) Species: 316 (Stutzerimonas stutzeri)

QKIHVGPGELDDYYGFWSGGHQGEVRVLGVPSMRELMRIPVFNVDSATGW
GLTNESRHIMGDSAKFLNGDCHHPHISMTDGKYDGKYLFINDKANSRVAR
IRLDIMKCDKMITVPNVQAIHGLRLQKVPHTKYVFANAEFIIPHPNDGKV
FDLQDENSYTMYNAIDAETMEMAFQVIVDGNLDNTDADYTGRFAAATCYN
SEKAFDLGGMMRNERDWVVVFDIHAVEAAVKAGDFITLGDSKTPVLDGRK
KDGKDSKFTRYVPVPKNPHGCNTSSDGKYFIAAGKLSPTCSMIAIDKLPD
LFAGKLADPRDVIVGEPELGLGPLHTTFDGRGNAYTTLFIDSQVVKWNME
EAVRAYKGEKVNYIKQKLDVHYQPGHLHASLCETNEADGKWLVALSKFSK
DRFLPVGPLHPENDQLIDISGDEMKLVHDGPTFAEPHDCIMARRDQIKTK
KIWDRNDPFFAPTVEMAKKDGINLDTDNKVIRDGNKVRVYMTSMAPAFGV
QEFTVKQGDEVTVTITNIDQIEDVSHGFVVVNHGVSMEISPQQTSSITFV
ADKPGLHWYYCSWFCHALHMEMVGRMMVEPA

3D structure

• PDB: 3sbr N2O binding at a [4Cu:2S] copper-sulphur cluster in nitrous oxide reductase.
• Chain: H
• Resolution: 2.24 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): M551 P553
• Catalytic site (residue number reindexed from 1): M494 P496
• Enzyme Commision number: 1.7.2.4: nitrous-oxide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CUA	H	C618 W620 C622 H626 M629	C561 W563 C565 H569 M572
BS02	CUK	H	H129 H130 H178 H326 H382 H433 H494	H72 H73 H121 H269 H325 H376 H437
BS03	CA	H	Y256 E259 M267 D273 N324	Y199 E202 M210 D216 N267
BS04	N2O	H	F621 H626 M627	F564 H569 M570

Gene Ontology

Molecular Function

• GO:0004129 cytochrome-c oxidase activity
• GO:0005507 copper ion binding
• GO:0005509 calcium ion binding
• GO:0050304 nitrous-oxide reductase activity

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:3sbr, PDBe:3sbr, PDBj:3sbr
• PDBsum: 3sbr
• PubMed: 21841804
• UniProt: P19573|NOSZ_STUST Nitrous-oxide reductase (Gene Name=nosZ)