Structure of PDB 3n7u Chain H

Receptor sequence

>3n7uH (length=351) Species: 3702 (Arabidopsis thaliana)

DSKKIVGVFYKANEYATKNPNFLGCVENALGIRDWLESQGHQYIVTDDKE
GPDCELEKHIPDLHVLISTPFHPAYVTAERIKKAKNLKLLLTAGIGSDHI
DLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVVKGEW
NVAGIAYRAYDLEGKTIGTVGAGRIGKLLLQRLKPFGCNLLYHDRLQMAP
ELEKETGAKFVEDLNEMLPKCDVIVINMPLTEKTRGMFNKELIGKLKKGV
LIVNNARGAIMERQAVVDAVESGHIGGYSGDVWDPQPAPKDHPWRYMPNQ
AMTPHTSGTTIDAQLRYAAGTKDMLERYFKGEDFPTENYIVKDGELAPQY
R

3D structure

• PDB: 3n7u Structures of the apo and holo forms of NAD-dependent formate dehydrogenase from the higher-plant Arabidopsis Thaliana
• Chain: H
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N146 R284 D308 Q313 H332
• Catalytic site (residue number reindexed from 1): N119 R257 D281 Q286 H305
• Enzyme Commision number: 1.17.1.9: formate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	H	I122 N146 V150 G200 R201 I202 D221 R222 M255 P256 N282 A283 R284 D308 H332 S334 G335 Q376	I95 N119 V123 G173 R174 I175 D194 R195 M228 P229 N255 A256 R257 D281 H305 S307 G308 Q349
BS02	AZI	H	P97 F98 I122 R284 H332	P70 F71 I95 R257 H305

Gene Ontology

Molecular Function

• GO:0008863 formate dehydrogenase (NAD+) activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0051287 NAD binding

Biological Process

• GO:0042183 formate catabolic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005829 cytosol
• GO:0009507 chloroplast
• GO:0009536 plastid
• GO:0009579 thylakoid

External links

• PDB: RCSB:3n7u, PDBe:3n7u, PDBj:3n7u
• PDBsum: 3n7u
• UniProt: Q9S7E4|FDH_ARATH Formate dehydrogenase, chloroplastic/mitochondrial (Gene Name=FDH1)