Structure of PDB 3kr4 Chain H

Receptor sequence

>3kr4H (length=509) Species: 36329 (Plasmodium falciparum 3D7)

SEVPQVVSLDPTSIPIEYNTPIHDIKVQVYDIKGGCNVEEGLTIFLVNNP
KENGPVKISSKVNDKQVSEFLKDENMEKFNVKLGTSKHFYMFNDNKNSVA
VGYVGCGSVADLSEADMKRVVLSLVTMLHDNKLSKLTVVFEINVDKNLFR
FFLETLFYEYMTDERFKSYIKHLGVYINNADTYKEEVEKARVYYFGTYYA
SQLIAAPSNYCNPVSLSNAAVELAQKLNLEYKILGVKELEELKMGAYLSV
GKGSMYPNKFIHLTYKSKGDVKKKIALVGKGITFDSGGYNLKAAPGSMID
LMKFDMSGCAAVLGCAYCVGTLKPENVEIHFLSAVCENMVSKNSYRPGDI
ITASNGKTIEVGNTDAEGRLTLADALVYAEKLGVDYIVDIATLTGAMLYS
LGTSYAGVFGNNEELINKILQSSKTSNEPVWWLPIINEYRATLNSKYADI
NQISSSVKASSIVASLFLKEFVQNTAWAHIDIAGVSWNFKARKPKGFGVR
LLTEFVLND

3D structure

• PDB: 3kr4 Structure of the Plasmodium falciparum M17 aminopeptidase and significance for the design of drugs targeting the neutral exopeptidases
• Chain: H
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K386 R463
• Catalytic site (residue number reindexed from 1): K292 R369
• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.
  3.4.13.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CO3	H	A460 G462 R463 L487	A366 G368 R369 L393
BS02	ZN	H	K374 D379 D399 E461	K280 D285 D305 E367
BS03	BES	H	K374 D379 M396 F398 D399 N457 D459 E461 T486 L487 G489	K280 D285 M302 F304 D305 N363 D365 E367 T392 L393 G395	MOAD: Ki=25nM
BS04	MG	H	D379 D459 E461	D285 D365 E367

Gene Ontology

Molecular Function

• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3kr4, PDBe:3kr4, PDBj:3kr4
• PDBsum: 3kr4
• PubMed: 20133789
• UniProt: Q8IL11|AMPL_PLAF7 Leucine aminopeptidase (Gene Name=LAP)