Structure of PDB 3jcm Chain H

Receptor sequence
>3jcmH (length=843) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
EHTIFTQLKKNIPKTRYNRDYMLSMANIPERIINVGVIGPLHSGKTSLMD
LLVIDSHKRIPDMSKNVELGWKPLRYLDNLKQEIDRGLSIKLNGSTLLCT
DLESKSRMINFLDAPGHVNFMDETAVALAASDLVLIVIDVVEGVTFVVEQ
LIKQSIKNNVAMCFVINKLDRLILDLKLPPMDAYLKLNHIIANINSFTKG
NVFSPIDNNIIFASTKLGFTFTIKEFVSYYYAHSIPSSKIDDFTTRLWGS
VYYHKGNFRTKPFENVEKYPTFVEFILIPLYKIFSYALSMEKDKLKNLLR
SNFRVNLSQEALQYDPQPFLKHVLQLIFRQQTGLVDAITRCYQPFELFDN
KTAHLSIPGKSTPEGTLWAHVLKTVDYGGAEWSLVRIYSGLLKRGDTVRI
LDTSQSESREDDETPSCEVEEIGLLGGRYVYPVHEAHKGQIVLIKGISSA
YIKSATLYSVKSKEDMKQLKFFKPLDYITEAVFKIVLQPLLPRELPKLLD
ALNKISKYYPGVIIKVEESGEHVILGNGELYMDCLLYDLRASYAKIEIKI
SDPLTVFSESCSNSRLGEENLPGLSISVAAEPMDSKMIQDLSRNTLGKGQ
NCLDIDGIMDNPRKLSKILRTEYGWDSLASRNVWSFYNGNVLINDTLPDE
ISPELLSKYKEQIIQGFYWAVKEGPLAEEPIYGVQYKLLSISVPSDVNID
VMKSQIIPLMKKACYVGLLTAIPILLEPIYEVDITVHAPLLPIVEELMKK
RRGSRIYKTIKVAGTPLLEVRGQVPVIESAGFETDLRLSTNGLGMCQLYF
WHKIWRKVPGDVLDKDAFIPKLKPAPINSLSRDFVMKTRRRKG
3D structure
PDB3jcm The 3.8 angstrom structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis
ChainH
Resolution3.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna H K111 K173 K182 I185 K10 K72 K81 I84
BS02 GTP H H143 S144 G145 K146 T147 S148 R176 L189 S190 K269 D271 S315 T316 K317 H42 S43 G44 K45 T46 S47 R75 L88 S89 K168 D170 S214 T215 K216
Gene Ontology
Molecular Function
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0030623 U5 snRNA binding
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000349 generation of catalytic spliceosome for first transesterification step
GO:0000387 spliceosomal snRNP assembly
GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071007 U2-type catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3jcm, PDBe:3jcm, PDBj:3jcm
PDBsum3jcm
PubMed26743623
UniProtP36048|SN114_YEAST Pre-mRNA-splicing factor SNU114 (Gene Name=SNU114)

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