Structure of PDB 3gli Chain H

Receptor sequence

>3gliH (length=365) Species: 83333 (Escherichia coli K-12)

QVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTS
IARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVED
TRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFL
LATTDPQKLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRAL
QLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLV
EAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALG
NDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTL
LRALAFHPRMPLPEP

3D structure

• PDB: 3gli The mechanism of ATP-dependent primer-template recognition by a clamp loader complex.
• Chain: H
• Resolution: 3.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	H	T99 K100 V101 S132 H134	T96 K97 V98 S129 H131	PDBbind-CN: Kd=0.38uM
BS02	peptide	H	Q296 L297 S298 P299 T323 Q326 Q330 T331 F359	Q293 L294 S295 P296 T320 Q323 Q327 T328 F356
BS03	ADP	H	A7 R11 P12 V19 G48 V49 G50 K51 T52 S53 L214 R215	A4 R8 P9 V16 G45 V46 G47 K48 T49 S50 L211 R212
BS04	BEF	H	R47 K51 E127 R215	R44 K48 E124 R212
BS05	ZN	H	C64 C79	C61 C76

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0006260 DNA replication

Cellular Component

• GO:0009360 DNA polymerase III complex

External links

• PDB: RCSB:3gli, PDBe:3gli, PDBj:3gli
• PDBsum: 3gli
• PubMed: 19450514
• UniProt: P06710|DPO3X_ECOLI DNA polymerase III subunit tau (Gene Name=dnaX)