Structure of PDB 3g1f Chain H

Receptor sequence
>3g1fH (length=216) Species: 187420 (Methanothermobacter thermautotrophicus str. Delta H) [Search protein sequence]
DVMNRLILAMDLMNRDDALRVTGEVREYIDTVKIGYPLVLSEGMDIIAEF
RKRFGCRIIADFKVADIPETNEKICRATFKAGADAIIVHGFPGADSVRAC
LNVAEEMGREVFLLTEMSHPGAEMFIQGAADEIARMGVDLGVKNYVGPST
RPERLSRLREIIGQDSFLISPGVGAQGGDPGETLRFADAIIVGRSIYLAD
NPAAAAAGIIESIKDL
3D structure
PDB3g1f Mechanism of the orotidine 5'-monophosphate decarboxylase-catalyzed reaction: evidence for substrate destabilization.
ChainH
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K42 D70 K72 D75
Catalytic site (residue number reindexed from 1) K33 D61 K63 D66
Enzyme Commision number 4.1.1.23: orotidine-5'-phosphate decarboxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 2OM H D75 I76 T79 D66 I67 T70 MOAD: Ki=100uM
BS02 2OM H D20 K42 D70 K72 M126 S127 P180 Q185 G202 R203 D11 K33 D61 K63 M117 S118 P171 Q176 G193 R194 MOAD: Ki=100uM
Gene Ontology
Molecular Function
GO:0004590 orotidine-5'-phosphate decarboxylase activity
GO:0016831 carboxy-lyase activity
Biological Process
GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
GO:0006221 pyrimidine nucleotide biosynthetic process
GO:0044205 'de novo' UMP biosynthetic process
Cellular Component
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:3g1f, PDBe:3g1f, PDBj:3g1f
PDBsum3g1f
PubMed19435314
UniProtO26232|PYRF_METTH Orotidine 5'-phosphate decarboxylase (Gene Name=pyrF)

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