Structure of PDB 2zhq Chain H

Receptor sequence

>2zhqH (length=250) Species: 9606 (Homo sapiens)

IVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPW
DKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDI
ALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKEGQPS
VLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGP
FVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFG

3D structure

• PDB: 2zhq Non-additivity of functional group contributions in protein-ligand binding: a comprehensive study by crystallography and isothermal titration calorimetry.
• Chain: H
• Resolution: 1.96 Å

Enzymatic activity

• Enzyme Commision number: 3.4.21.5: thrombin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	H	E23 I24 G25 M26 W29 F114 D116 H119 P120 C122 Y134 K135 R137 N159 M201 K202 N205 R206 W207	E8 I9 G10 M11 W14 F111 D113 H116 P117 C119 Y134 K135 R137 N156 M203 K204 N209 R210 W211
BS02	peptide	H	F34 Q38 L40 L65 R73 T74 R75 Y76 I82 M84	F19 Q24 L26 L60 R68 T69 R70 Y71 I78 M80
BS03	27U	H	H57 Y60A L99 D189 A190 V213 W215 G216	H43 Y47 L96 D191 A192 V217 W219 G220	MOAD: Ki=0.75uM BindingDB: Ki=220nM

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0005509 calcium ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0007596 blood coagulation

External links

• PDB: RCSB:2zhq, PDBe:2zhq, PDBj:2zhq
• PDBsum: 2zhq
• PubMed: 20156458
• UniProt: P00734|THRB_HUMAN Prothrombin (Gene Name=F2)