Structure of PDB 1r4n Chain H

Receptor sequence
>1r4nH (length=418) Species: 9606 (Homo sapiens) [Search protein sequence]
DWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGC
ELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFL
NDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISL
LNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQV
NFPMATIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIF
QKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIA
TSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKL
QEVLDYLTNSASLQMKSPAITATNRTLYLQSVTSIEERTRKELGLVDGQE
AVADVTTPQTVLFKLHFT
3D structure
PDB1r4n The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1.
ChainH
Resolution3.6 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) K21 D146 A216 T217 A219
Catalytic site (residue number reindexed from 1) K10 D135 A205 T206 A208
Enzyme Commision number 6.2.1.64: E1 NEDD8-activating enzyme.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN H C199 C343 C346 C188 C332 C335
BS02 ATP H D79 M80 D81 R90 Q91 K103 K126 I127 Q128 L145 D146 D68 M69 D70 R79 Q80 K92 K115 I116 Q117 L134 D135
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008641 ubiquitin-like modifier activating enzyme activity
GO:0016874 ligase activity
GO:0019781 NEDD8 activating enzyme activity
GO:0019788 NEDD8 transferase activity
GO:0042802 identical protein binding
GO:0046982 protein heterodimerization activity
Biological Process
GO:0000278 mitotic cell cycle
GO:0006508 proteolysis
GO:0007113 endomitotic cell cycle
GO:0036211 protein modification process
GO:0043687 post-translational protein modification
GO:0045116 protein neddylation
GO:0051726 regulation of cell cycle
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0032991 protein-containing complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:1r4n, PDBe:1r4n, PDBj:1r4n
PDBsum1r4n
PubMed14690597
UniProtQ8TBC4|UBA3_HUMAN NEDD8-activating enzyme E1 catalytic subunit (Gene Name=UBA3)

[Back to BioLiP]