Structure of PDB 1hr9 Chain H

Receptor sequence

>1hr9H (length=440) Species: 4932 (Saccharomyces cerevisiae)

IPGTRTSKLPNGLTIATEYIPNTSSATVGIFVDAGSRAENVKNNGTAHFL
QHLAFKGTQNRPQQGIELEIENIGSHLNAYTSRENTVYYAKSLQEDIPKA
VDILSDILTKSVLDNSAIERERDVIIRESEEVDKMYDEVVFDHLHEITYK
DQPLGRTILGPIKNIKSITRTDLKDYITKNYKGDRMVLAGAGAVDHEKLV
QYAQKYFGHVPKSESPVPLGSPRGPLPVFCRGERFIKENTLPTTHIAIAL
EGVSWSAPDYFVALATQAIVGNWDRAIGTGTNSPSPLAVAASQNGSLANS
YMSFSTSYADSGLWGMYIVTDSNEHNVRLIVNEILKEWKRIKSGKISDAE
VNRAKAQLKAALLLSLDGSTAIVEDIGRQVVTTGKRLSPEEVFEQVDKIT
KDDIIMWANYRLQNKPVSMVALGNTSTVPNVSYIEEKLNQ

3D structure

• PDB: 1hr9 Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences.
• Chain: H
• Resolution: 3.01 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H70 Q73 H74 E143 E150
• Catalytic site (residue number reindexed from 1): H48 Q51 H52 E121 E128
• Enzyme Commision number: 3.4.24.64: mitochondrial processing peptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	H	Q73 A101 Y102 T103 E150 V154 E160 W277 F326 S327	Q51 A79 Y80 T81 E128 V132 E138 W255 F304 S305
BS02	ZN	H	H70 H74 E150	H48 H52 E128

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0005515 protein binding
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0006627 protein processing involved in protein targeting to mitochondrion

Cellular Component

• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0017087 mitochondrial processing peptidase complex

External links

• PDB: RCSB:1hr9, PDBe:1hr9, PDBj:1hr9
• PDBsum: 1hr9
• PubMed: 11470436
• UniProt: P10507|MPPB_YEAST Mitochondrial-processing peptidase subunit beta (Gene Name=MAS1)