Structure of PDB 1gq2 Chain H

Receptor sequence

>1gq2H (length=555) Species: 8932 (Columba livia)

KKGYEVLRDPHLNKGMAFTLEERQQLNIHGLLPPCFLGQDAQVYSILKNF
ERLTSDLDRYILLMSLQDRNEKLFYKVLTSDIERFMPIVYTPTVGLACQH
YGLAFRRPRGLFITIHDRGHIATMLQSWPESVIKAIVVTDGERILGLGDL
GCYGMGIPVGKLALYTACGGVKPHQCLPVMLDVGTDNETLLKDPLYIGLR
HKRIRGQAYDDLLDEFMEAVTSRYGMNCLIQFEDFANANAFRLLHKYRNK
YCTFNDDIQGTASVAVAGLLAALRITKNRLSDHTVLFQGAGEAALGIANL
IVMAMQKEGVSKEEAIKRIWMVDSKGLIVKGRASLTPEKEHFAHEHCEMK
NLEDIVKDIKPTVLIGVAAIGGAFTQQILQDMAAFNKRPIIFALSNPTSK
AECTAEQLYKYTEGRGIFASGSPFDPVTLPSGQTLYPGQGNNSYVFPGVA
LGVISCGLKHIGDDVFLTTAEVIAQEVSEENLQEGRLYPPLVTIQQVSLK
IAVRIAKEAYRNNTASTYPQPEDLEAFIRSQVYSTDYNCFVADSYTWPEE
AMKVK

3D structure

• PDB: 1gq2 Structural Studies of the Pigeon Cytosolic Nadp+ -Dependent Malic Enzyme
• Chain: H
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y112 R165 K183 E255 D256 D278 D279 N421
• Catalytic site (residue number reindexed from 1): Y90 R143 K161 E233 D234 D256 D257 N396
• Enzyme Commision number: 1.1.1.40: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAP	H	R165 L167 G168 N259 T283 Q310 A312 G313 E314 A315 D345 S346 K362 A393 L419 N421 G465 N466 N467	R143 L145 G146 N237 T261 Q288 A290 G291 E292 A293 D323 S324 K339 A368 L394 N396 G440 N441 N442
BS02	OXL	H	R165 K183 N421 N467	R143 K161 N396 N442
BS03	MN	H	E255 D256 D279	E233 D234 D257

Gene Ontology

Molecular Function

• GO:0004470 malic enzyme activity
• GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
• GO:0008948 oxaloacetate decarboxylase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0046872 metal ion binding
• GO:0051287 NAD binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006108 malate metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion

External links

• PDB: RCSB:1gq2, PDBe:1gq2, PDBj:1gq2
• PDBsum: 1gq2
• PubMed: 11790843
• UniProt: P40927|MAOX_COLLI NADP-dependent malic enzyme (Gene Name=ME1)