Structure of PDB 8wib Chain G

Receptor sequence

>8wibG (length=208) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

LKVDVKTPAGKTDGSVELPAELFDVEPNIALMHQVVTAQLAAKRQGTHST
KTRGEVSGGGKKPYRQKGTGRARQGSTRAPQFTGGGTVHGPKPRDYSQRT
PKKMIAAALRGALSDRARNDRIHAVTELVEGQTPSTKSAKTFLGTLTENK
KVLVVIGRTDEVGAKSVRNLPGVHVISPDQLNTYDVLNADDVVFSVEALN
AYISANSK

3D structure

• PDB: 8wib Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
• Chain: G
• Resolution: 3.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	G	N30 H35 Q36 Q41 L42 K45 R46 Q47 T49 H50 S51 T52 K53 R55 V58 S59 G60 G62 K63 K64 Q68 K69 R73 R75 G77 R80 Q83 T85 G86 T89 V90 H91 P93 P95 Q100 R101 K104 K105 I107 P136 T138 K139 K153 R160 K167 R170 N171 H176 I178 Q182 N184 Y186 D187	N28 H33 Q34 Q39 L40 K43 R44 Q45 T47 H48 S49 T50 K51 R53 V56 S57 G58 G60 K61 K62 Q66 K67 R71 R73 G75 R78 Q81 T83 G84 T87 V88 H89 P91 P93 Q98 R99 K102 K103 I105 P134 T136 K137 K151 R158 K165 R168 N169 H174 I176 Q180 N182 Y184 D185

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8wib, PDBe:8wib, PDBj:8wib
• PDBsum: 8wib
• PubMed: 38245551
• UniProt: A0QSD2|RL4_MYCS2 Large ribosomal subunit protein uL4 (Gene Name=rplD)