Structure of PDB 8t7s Chain G

Receptor sequence

>8t7sG (length=1157) Species: 1314 (Streptococcus pyogenes)

KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILL
SDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFF
DQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQ
RTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYV
GPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNL
PNEKVLPKHSLLYEYFTVYNELTEQKKAIVDLLFKTNRKVTVKQLKEDYF
KKRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIE
ERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFL
KSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAI
KKGILQTVKVVDELVKVMGRHKPENIVIEMAREGLSELDKAGFIKRQLVE
TRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY
KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYYSNIMNFFKTEIT
LANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQ
TGGFSKESIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEK
GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKY
SLFELENGRKRMLASAKQLQKGNELALPSKYVNFLYLASHYENEQKQLFV
EQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENI
IHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLDATLIHQSITGLYETR
IDLSQLG

3D structure

• PDB: 8t7s Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
• Chain: G
• Resolution: 3.01 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	G	R1114 L1135 W1136 K1200 A1215 S1216 Q1219 Q1221 A1320 R1322 Q1335 R1337	R913 L934 W935 K999 A1014 S1015 Q1018 Q1020 A1111 R1113 Q1126 R1128
BS02	rna	G	K30 K33 L35 R40 I43 K44 N46 G56 E57 T58 A59 R61 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 N77 R78 Y81 L101 S104 F105 R115 H116 P117 I122 E125 V126 H129 T134 I135 H160 K163 F164 R165 G166 Y325 H328 H329 K336 Y347 F351 F352 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 R467 T472 I473 P475 M495 K735 K742 K749 R753 M1089 P1090 Q1091 K1097 E1099 V1100 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 W1126 K1130 Y1131 M1169 S1172 K1211 E1225 L1226 A1227 Q1272 H1349 Q1350 S1351 Y1356 E1357 T1358	K28 K31 L33 R38 I41 K42 N44 G54 E55 T56 A57 R59 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 N75 R76 Y79 L99 S102 F103 R113 H114 P115 I120 E123 V124 H127 T132 I133 H158 K161 F162 R163 G164 Y323 H326 H327 K334 Y345 F349 F350 I361 Q400 R401 T402 F403 N405 G406 F444 R445 I446 Y448 G451 P452 L453 A454 R455 N457 S458 F460 W462 R465 T470 I471 P473 M493 K702 K709 K716 R720 M888 P889 Q890 K896 E898 V899 T901 G902 G903 F904 I909 P911 K912 R921 K922 K923 W925 K929 Y930 M968 S971 K1010 E1024 L1025 A1026 Q1063 H1140 Q1141 S1142 Y1147 E1148 T1149
BS03	dna	G	K1107 E1108 W1136	K906 E907 W935

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:8t7s, PDBe:8t7s, PDBj:8t7s
• PDBsum: 8t7s
• PubMed: 38688943
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)